大鼠坐骨神经夹伤过程中腓肠肌组织Kif11和Kif15基因的表达变化  

Expression of Kif11 and Kif15 mRNA in rat gastrocnemius after sciatic nerve crushed

作　　者：巫荣华[1] 陆伟[1] 陈海姣[1] 刘梅[1] 

机构地区：[1]南通大学神经再生重点实验室,南通226001

出　　处：《南通大学学报（医学版）》2010年第4期235-238,241,共5页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金(30600172);教育部科学技术研究重点项目(208044)

摘　　要：目的:研究驱动蛋白家族成员11(kinesin family member 11,Kif11)和驱动蛋白家族成员15(kinesin familymember 15,Kif15)在大鼠失神经肌萎缩及再支配过程中的表达变化。方法:建立大鼠坐骨神经夹伤模型,测定腓肠肌湿重比了解肌萎缩程度,采用足迹实验测定坐骨神经功能指数了解神经损伤再支配恢复情况,采用Real-time PCR方法检测坐骨神经夹伤后不同时间腓肠肌组织中Kif11和Kif15 mRNA水平的表达变化。结果:在神经夹伤早期(1～7 d),腓肠肌组织中的Kif11和Kif15 mRNA迅速上升,7～14 d达到高峰,随后mRNA水平逐渐下降,至28 d时基本接近正常表达水平;同时可观察到腓肠肌湿重比及坐骨神经功能指数的恢复。结论:Kif11和Kif15 mRNA的变化与坐骨神经损伤(夹伤)引起的肌萎缩程度呈正相关。Objective： To investigate the expression pattern of kinesin family member 11（Kif11） and kinesin family member 15（Kif15） in rat gastrocnemius after sciatic nerve crushed.Methods： The Kif11 and Kif15 mRNA were conducted by quantitative real-time PCR.The ratio of muscle wet weight and sciatic functional index（SFI） were measured.Results： The expression levels of Kif11 and Kif15 mRNA in gastrocnemius were increased instantly in the early stage（1～7 d） after sciatic nerve crushed.Then,they reached to the peak from 7 d to 14 d,followed by decreased gradually after 14 d and closed to the normal expression levels at 28 d.At the same time,the recovery of the ratio of muscle wet weight and SFI was observed.Conclusion： The results of this study provided evidence for a significant positive correlation between Kif11,Kif15 expression and muscle atrophy within a 28-day period after nerve injury.This suggested that Kif11 and Kif15 might play an important role in the rat gastrocnemius atrophy and regeneration.

关 键 词：坐骨神经夹伤 驱动蛋白家族成员11 驱动蛋白家族成员15 肌萎缩 

分 类 号：R651.3[医药卫生—外科学]