基于代谢组学技术的2,4-二硝基苯酚和干酵母致热病证候模型的对比研究  

A comparative study of the fever syndrome model induced with 2,4-dinitrophenol and dry yeast based on metabonomics

作　　者：黄志桓[1] 柳长凤[1] 刘树民[1] 李俊行[1] 汪娜[1] 祖金祥[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《中药药理与临床》2010年第3期61-65,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家重点基础研究发展计划973计划(编号:2007CB512608)

摘　　要：目的:基于代谢组学理论,探讨2,4-二硝基苯酚和干酵母致热病证候模型在代谢组学层面上的关联性。方法:运用UPLC-Q-TOF/MS技术手段,利用PCA和PLS-DA数据解析方法,研究正常大鼠、2,4-二硝基苯酚致热病证候模型大鼠和干酵母致热病证候模型大鼠尿液中代谢物组时量的轨迹变化和对应关系。结果:初步确定了两个热病证候模型相同的生物标志物的变化规律存在差异。结论:应用代谢组学方法,对比两个热病证候模型的生物标志物的变化规律,阐明了两个热病证候模型的差异性。Objective：To explore the correlation between 2,4-dinitrophenol-induced and dry yeast-induced fever syndrome model based on metabonomics.Method：The metabolites in urine of the control、2,4-dinitrophenol-induced and dry yeast-induced fever syndrome model rats were determined by UPLC-Q-TOF/MS and then analyzed by PCA and PLS-DA.Results：The differences of the variations of the same biomarkers were preliminarily found in the two fever Syndrome model.Conclusion：We clarified the differences between the two fever Syndrome model after comparing the biomarker variations by means of metabonomics.

关 键 词：2 4-二硝基苯酚 干酵母 代谢组学 生物标记物 热病证候 

分 类 号：R-332[医药卫生]