转双反义SBE基因大米的亚慢性毒性实验  

Subchronic toxicity test of genetically modified rice with double antisense starch-branching enzyme gene

作　　者：李敏[1] 朴建华[1] 杨晓光[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所卫生部微量元素营养重点实验室,北京100050

出　　处：《卫生研究》2010年第4期436-439,443,共5页Journal of Hygiene Research

基　　金：转基因产品食用安全性评价模型(No.2007CB109207)

摘　　要：目的观察转双反义SBE基因大米是否具有潜在的毒副作用。方法初断乳Wistar大鼠按性别、体重随机分为5组,分别喂饲高剂量亲本大米饲料(A组)、高剂量转基因大米饲料(B组)、中剂量转基因大米饲料(C组)、低剂量转基因大米饲料(D组)和AIN-93G正常对照饲料(E组)。所有动物自由进食饮水,观察大鼠体重、进食量、血常规、血生化、脏器重量、骨密度以及脏器的病理学检查。结果实验中期,B组雌性大鼠的单核细胞百分比显著低于E组(P<0.05),谷草转氨酶活性显著高于E组(P<0.05)。C组雌性大鼠的谷丙转氨酶活性显著高于A组和E组(P<0.05)。实验末期,B组雌性大鼠的谷丙转氨酶和谷草转氨酶活性高于E组(P<0.05)。B组雄性大鼠红细胞压积和尿素氮显著低于亲A组(P<0.05),E组单核细胞百分比显著高于(P<0.05)。B组雌性大鼠大脑的脏器系数显著大于A组(P<0.05),B、C、D组雌性大鼠的肾脏脏器系数显著小于E组(P<0.05)。实验大鼠各组之间血脂、血钙和骨密度均无统计学差异,内脏病理检查未发现明显异常。结论现有实验结果不能证实该转基因大米对大鼠有亚慢性毒性作用。Objective To observe the sub-chronic toxic effects of the genetically modified rice with double antisense SBE gene.Methods Based on gender and weight,weanling Wistar rats were randomly sorted into five groups： non-genetically modified rice group（group A）,genetically modified rice group （group B）,half genetically modified rice group（ group C）,quarter genetically modified rice group（ group D） and AIN-93G normal diet group （group E）.Indicators were the followings： body weight,food consumption,blood routine,blood biochemical test, organ weight,bone density and pathological examination of organs.Results At the middle of the experiment,the percentage of monocyte of female group B was less than that of group E （P〈0.05）.AST activity of female group B was higher than that of group E （P〈0.05）.ALT activity of female group C was higher than that of groups A and E （P〈0.05）.At the end of the experiment,AST and ALT activities of female group B were higher than those of group E （P〈0.05）.Hematocrit （HCT） and blood urea nitrogen （ BUN） level of male group B were less than those of group A （P〈0.05）,and monocyte was higher than that of group E （ P〈0.05）.The brain index of female group B was higher than group A （ P〈0.05） and the kidney index of group E were higher than those of groups B,C and D （P〈0.05）.There were no significant difference of blood lipids,calcium and bone mineral density among all groups （P〉0.05）,and no notable abnormity in the pathological examination of main organs （ P〈0.05）.Conclusion There were no enough evidence to confirm the sub-chronic toxicity of genetically modified rice on rats.

关 键 词：转基因大米 SBE基因 亚慢性毒性 食品安全 

分 类 号：R155.1[医药卫生—营养与食品卫生学] TS201.6[医药卫生—公共卫生与预防医学]