海洋小链霉菌DY2741抗菌物质的溶解性质及分离纯化  

Isolation,Purification and Solubility of Antibacterial Substances from Fermentation Supernatant of Marine Streptomyces parvus DY2741

作　　者：刘伟[1,2] 徐涛[2] 蔡敬民[1,2] 潘仁瑞[2] 吴缨[3] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]合肥学院生物与环境工程系,安徽合肥230022 [3]合肥学院化学与材料工程系,安徽合肥230022

出　　处：《食品科学》2010年第15期177-180,共4页Food Science

基　　金：安徽省优秀青年科技基金项目(04043051);国家自然科学基金项目(30270321);合肥学院(引进)人才科研启动基金计划项目(08RC12);安徽省教育厅自然科学研究项目(KJ2008B229)

摘　　要：在拮抗金黄色葡萄球菌和大肠杆菌活性的指示下将一株海洋小链霉菌DY2741的代谢产物分离纯化。通过对该菌发酵液中抗菌物质的溶解性质测定,可推知该物质极性中等。Sephadex LH-20柱色谱分离后,经抗菌活性检测发现两个活性峰,其中第2个峰的抑菌活性较强。将第2个峰的洗脱液各组分进行薄层色谱分析,选取斑点清晰且单一的组分进行气相色谱-质谱联用分析,总离子流图表明该组分成分单一,根据质谱图谱与标准谱库比对以及红外光谱分析,初步判断该组分为邻苯二甲酸二丁酯(DBP)。该成分为小链霉菌DY2741的主要抗菌物质之一。One aliquot of the fermentation supernatant of Streptomyces parvus DY2741 using soybean powder as substrate was vacuum dried and the residue was extracted with water or 5 different organic solvent alone.The ethyl acetate extract was found to have good inhibitory activity against both S.aureus and E.coli.Another aliquot of the fermentation supernatant was directly extracted with ethyl acetate 3 times and the extracts were then pooled and condensed in a rotation evaporator and blown to dryness under nitrogen.Finally,the residue was dissolved in methanol and filtrated before purification/fractionization on Sephadex LH-20 column.As a result,two antibacterial fractions were obtained and the second eluting peak presented stronger inhibitory activities against both bacterial species.Thin layer chromatography was used to further fractionize the fraction with higher antibacterial activity.The subfaraction displaying a clear single spot was demonstrated by gas chromatography-mass spectrometry to contain a single composition,which was preliminarily identified to be dibutyl phthalate（DBP） based on searching Wiley Registry of Mass Spectral Data and infrared spectral analysis.Therefore,DBP is one of the major antibacterial substances produced by Streptomyces parvus DY2741.

关 键 词：海洋小链霉菌 抗菌物质 溶解性质 分离纯化 

分 类 号：R282.77[医药卫生—中药学]