荷叶离褶伞液体发酵及生物酶法提取菌丝体多糖的研究  

Fermentation of Lyophyllum decastes and Extraction of Polysaccharides from Mycelia by Enzymatic Hydrolysis

作　　者：闫培生[1] 马丽雅[1] 朱国振[1] 薛梅[1] 

机构地区：[1]哈尔滨工业大学(威海)海洋学院,山东威海264209

出　　处：《中国农业科技导报》2010年第4期84-89,共6页Journal of Agricultural Science and Technology

基　　金：国家863计划项目(2008AA10Z327);哈尔滨工业大学优秀创新团队计划资助

摘　　要：首次利用10 L发酵罐研究了荷叶离褶伞菌丝生长、胞内和胞外多糖产量,以及6种酶对发酵菌丝体多糖的提取效果。结果表明,在25℃、120 r/min,空气流量为200 L/h的条件下发酵,8 d时胞内多糖含量(63.1 mg/g干菌丝体)和胞外多糖产量(3.54 g/L)达最高,9 d时菌丝体产量(17.86 g/L)及胞内多糖产量(1.11 g/L)达最高,与摇瓶发酵相比,其产量均显著提高。参试的6种酶中,溶壁酶酶解处理菌丝体获得的多糖产量最高,而中性蛋白酶是最经济适宜的酶类,与不加酶的水提法相比,多糖提取率分别提高了459.29%和376.11%,中性蛋白酶酶解制备菌丝体多糖的适宜条件为:在1%酶用量下,30℃酶解2 h后,95℃提取2次,每次2 h。The mycelial growth,intro-and extro-polysaccharide production of Lyophyllum decastes were investigated in this research using a 10 L fermentor,and the effects of six enzymes on extraction of mycelial intro-polysaccharide were also studied.The results indicated that under conditions of 25℃,120 r/min and 200L air flux /h,the content of intro-polysaccharide and yield of extro-polysaccharide could reach the highest on day 8th with 63.1 mg/g dry mycelium and 3.54 g/L,respectively.But,the yields of mycelia and intro-polysaccharide could reach the maximum on the 9th day with 17.86 g/L and 1.11 g/L,respectively.These yields were significantly higher than that by using shaking flask.Among the 6 tested enzymes,the highest yield was obtained by lywallzyme for intro-polysaccharide extraction from mycelia,whereas neutral protease was the most economic enzyme.Comparing with hot-water treatment,the extraction rate was increased by 459.29% and 376.11%,respectively for lywallzyme and neutral protease.The optimal condition for preparation of mycelial polysaccharide by neutral protease was using 1% enzyme,incubated for 2 h at 30℃ during hydrolytic reaction,then followed by 2 times of the same steps for extraction at 95℃,each time for 2 h.

关 键 词：荷叶离褶伞 液体发酵 生物酶 多糖提取 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]