邻苯二甲酸二异丁酯对雄性小鼠空间学习记忆行为的影响  

Effects of Diisobutyl Phthalate (DiBP) on the Behavior of Male Mice in Spatial Learning and Memory

作　　者：马宁[1] 支媛[1] 徐海滨[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2010年第4期300-305,共6页Chinese Journal of Food Hygiene

基　　金：国家科技支撑计划课题(2006BAK02A07)

摘　　要：目的观察邻苯二甲酸二异丁酯(DiBP)经口亚慢性暴露,对雄性小鼠空间学习记忆行为的影响。方法每组12只雄性昆明小鼠,空白对照组和阳性对照组给予玉米油溶剂灌胃,各剂量组给予相应剂量的邻苯二甲酸二异丁酯(50、250、500、1000mg/kg BW)玉米油溶液灌胃,连续8周。染毒前后分别对各组动物进行旷场试验和Morris水迷宫试验,其中阳性对照组在染毒结束后进行旷场试验和Morris水迷宫试验前经腹腔注射5mg/kg BW的氢溴酸东莨菪碱。结果试验前各组动物的学习记忆能力一致。8周实验结束后,阳性对照组和1000mg/kg BW剂量组小鼠与空白对照组小鼠相比在旷场中心区域停留时间明显延长(P<0.05)。500mg/kg BW剂量组和阳性对照组与空白对照组相比Morris水迷宫训练第一天的逃避潜伏期明显延长(P<0.01),1000mg/kg BW剂量组与空白对照组相比逃避潜伏期也明显延长(P<0.05)。其他各项指标与空白对照组相比,差异均无统计学意义。结论在本试验条件下,DiBP会降低雄性昆明小鼠的空间学习记忆能力。Objective To study the effects of diisobutyl phthalate （DiBP） on the behavior of male mice in spatial learning and memory.Method Seventy two Kunming mice were randomly divided into six groups （12 mice in each group）,including solvent control group,positive control group （scopolamine hydrobromide） and four DiBP-treated groups.The mice in solvent and positive control group were given corn oil by gavages.The mice in DiBP-treated groups were given DiBP in corn oil by gavages at the dosage of 50,250,500 and 1 000 mg /kg BW respectively for 8 weeks.Morris water maze and open field tests were conducted at the beginning and by the end of the experiment.Mice in positive control group were injected with scopolamine hydrobromide （5 mg /kg BW） intraperitoneally before the last test.Results No significant difference in learning and memory ability of each group was observed at the beginning of the experiment.By the end of 8week experiment,the time of staying in the center of open field was longer in the positive control group and 1 000 mg /kg BW DiBP-treated group than that in solvent control group （P0.05） in the open field test.The latent time of finding a hidden platform on the first day of training was significantly increased in the positive control group and the 500 mg /kg BW and 1 000 mg /kg DiBP-treated groups in comparison with the solvent control group （P0.01） in the water maze test.Conclusion The spatial learning and memory ability of male kunming mice could be decreased on exposure to DiBP under the condition of this experiment.

关 键 词：邻苯二甲酸二异丁酯 旷场试验箱 MORRIS水迷宫 学习记忆 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]