微小亚历山大藻对黑鲷仔鱼的抗氧化酶和ATPase的胁迫影响  

Effects of Alexandrium minitum on the activities of the antioxidant system and ATPase of larvae of Sparus macrocephalus.

作　　者：蒋玫[1] 黄世林[2] 伦凤霞 夏培艳[4] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]国家海洋局第二海洋研究所,浙江杭州310012 [3]上海市水文总站,上海20023 [4]上海海洋大学,上海200336

出　　处：《海洋通报》2010年第4期427-431,共5页Marine Science Bulletin

基　　金：国家重点基础研究发展规划"973"项目资助(G2010CB428705);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)(2007M08);上海市科委"登山行动计划"项目(06dz2015)

摘　　要：研究了暴露于微小亚历山大藻藻液中的黑鲷仔鱼其超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化物酶(CAT)和Na+-K+-ATPase酶活性在4d时间内的变化,结果表明,微小亚历山大藻藻液对仔鱼的GSH-PX和CAT活性诱导作用明显,尤其对处于染毒时间2d内,这种作用较强,但随着实验时间的延长又表现出抑制作用。微小亚历山大藻藻液对SOD也有强烈的诱导作用,这种作用可持续3d,但随后则表现为抑制作用。仔鱼的3种酶活性与染毒的藻液均符合较明显的剂量-效应关系,即实验藻液浓度越大,对酶活性的抑制率越低,对生物毒性越大。但藻液对于Na+-K+-ATPase酶却表现出强烈的抑制作用,显示出浓度-效应关系。其与上述3种酶的活力变化可以一起作为水体中藻液污染的生物指示器。In this article, we studied the change of superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX）, peroxidase （CAT） and Na＋-K＋-ATPase activity of larvae of Sparus macrocephalus exposed to Alexandrium minitum algae liquid. It revealed that Alexandrium minitum algae liquid had obvious induction to the GSH-PX and CAT of larvae of Sparus macrocephalus in the early exposure stage, especially for the first two days. However, it showed inhibition effect in the following experiment after 2 days. Besides, Alexandrium minitum algae liquid showed strong induction effect to SOD for the first 3 days then changed to the inhibition effect. The obvious dose-response link between the concentration of exposed algae solution and the activity of SOD, GSH-PX and CAT showed that the higher the concentration of algae solution, the lower the inhibition and the greater the biological toxicity. However, the strong inhibition of algae solution to Na^＋-K^＋-ATPase displayed concentration-response link. The four enzyme’s activity changes of the larvae of Sparus macrocephalus studied in this paper can work as the biological indicator of algae pollution in water.

关 键 词：微小亚历山大藻 仔鱼 抗氧化酶 ATPASE 

分 类 号：S965.3[农业科学—水产养殖] Q143[农业科学—水产科学]