Self-assembly of Liposomes Loading Hinesol by Rapid Expansion from Supercritical to Surfactant Solution  

作　　者：WEN Zhen YOU Xin-kui LIU Bo ZHENG Zong-kun PU Yi-tao LI Qiong 

机构地区：[1]School of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, P. R. China [2]School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第4期617-621,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.20606013);the Produce-learn-research Project of Shenzhen Scinence and Technology Program(No.2008342);the Fundamental Research Project of Shenzhen Science and Technology, China(No.JC200903120089A)

摘　　要：To improve the encapsulation efficiency and minimize particle agglomeration of liposomes, rapid expansion from supercritical to surfactant solution（RESSS） was applied to self-assembly of liposomes entrapping hinesol in supercritical carbon dioxide（SC-CO2） medium. In the process, the solution of liposomal materials and hinesol dissolved in the mixture of SC-CO2/ethanol was sprayed into a surfactant solution. The surfactant provided stabilization for liposomes by preventing bubbles overflowing and liposomes agglomeration during the spraying process. The encapsulating performance and particle size distribution of liposomes could be controlled by changing expansion processing conditions. When the solution was sprayed into a 5.0% Poloxamer 188 solution at a flow rate of 2 L/min, the entrapment efficiency and average particle size of liposomes were found to be 88.26% and 124 nm, respectively, which came to the standards of Chinese pharmacopoeia. The results show RESSS process provides an innovative method for formation of liposomes incorporating valid compositions extracted from the Chinese traditional medicines in SC-CO2 medium.To improve the encapsulation efficiency and minimize particle agglomeration of liposomes, rapid expansion from supercritical to surfactant solution（RESSS） was applied to self-assembly of liposomes entrapping hinesol in supercritical carbon dioxide（SC-CO2） medium. In the process, the solution of liposomal materials and hinesol dissolved in the mixture of SC-CO2/ethanol was sprayed into a surfactant solution. The surfactant provided stabilization for liposomes by preventing bubbles overflowing and liposomes agglomeration during the spraying process. The encapsulating performance and particle size distribution of liposomes could be controlled by changing expansion processing conditions. When the solution was sprayed into a 5.0% Poloxamer 188 solution at a flow rate of 2 L/min, the entrapment efficiency and average particle size of liposomes were found to be 88.26% and 124 nm, respectively, which came to the standards of Chinese pharmacopoeia. The results show RESSS process provides an innovative method for formation of liposomes incorporating valid compositions extracted from the Chinese traditional medicines in SC-CO2 medium.

关 键 词：LIPOSOME HINESOL Supercritical carbon dioxide Rapid expansion SURFACTANT 

分 类 号：TQ460.4[化学工程—制药化工] O647.2[理学—物理化学]