气相色谱和红外光谱对金针菇多糖的分析研究  

Analysis of Flammalina velutipes polysaccharide by gas chromatography and infrared spectra

作　　者：李健[1] 张竹青[1] 陈辉[2] 

机构地区：[1]长春工业大学化学工程学院,吉林长春130012 [2]浙江理工大学理学院,浙江杭州310018

出　　处：《食品工业科技》2010年第9期147-149,共3页Science and Technology of Food Industry

摘　　要：采用乙醇沉淀得到金针菇多糖(FVP),用sevag法除去蛋白后采用气相色谱(GC)和红外光谱(IR)对得到的金针菇胞外及胞内多糖进行分析。结果表明,金针菇胞外多糖中含有鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖,其摩尔百分比为6·04%,8·79%,14·54%,22·56%,21·49%,26·58%;胞内多糖中含有鼠李糖、阿拉伯糖、木糖、甘露糖和葡萄糖,其摩尔百分比为1·88%,2·82%,5·00%,21·24%,69·06%。红外光谱分析金针菇胞外和胞内多糖中存在吡喃环和呋喃环。Flammalina velutipes polysaccharide（FVP）was obtained by ethanol precipitation.The protein was removed using sevag method.Flammalina velutipes exo-polysaccharide and intropolysaccharide were analyzed by gas chromatography（GC）and infrared spectra（IR）.The results showed that the monosaccharide composition of Flammalina velutipes exo-polysaccharide were rhamnose,arabinose,xylose,mannose,glucose and galactose with the molar percentage of 6.04%,8.79%,14.54%,22.56%,21.49%,26.58%.The monosaccharide composition of Flammalina velutipes intropolysaccharide were rhamnose,arabinose,xylose,mannose and glucose with the molar percentage of 1.88%,2.82%,5.00%,21.24%,69.06%.The IR results showed that there were pyran and furan ring existing in Flammalina velutipes exo-polysaccharide and intropolysaccharide.

关 键 词：金针菇多糖(FVP) 气相色谱(GC) 红外光谱(IR) 单糖组成 结构分析 

分 类 号：TS201.23[轻工技术与工程—食品科学]