高温短小芽孢杆菌及其产高温淀粉酶的初步研究  

Study on a thermophilic Bacillus pumilus sp. and its thermostable α-amylase

作　　者：丁必权[1] 李秋鹏[1] 季秀玲[1] 廖艳江[1] 魏云林[1] 林连兵[1] 

机构地区：[1]昆明理工大学生物工程技术研究中心,云南昆明650224

出　　处：《食品工业科技》2010年第9期185-187,190,共4页Science and Technology of Food Industry

基　　金：中国科学院微生物研究所微生物资源前期开发国家重点实验室开放课题的资助(SKLMR-080605);国家自然科学基金项目资助(30660009;30960022)

摘　　要：从云南腾冲热海热泉中分离出一株产高温淀粉酶的菌株,命名为Tamy12。该菌株生长温度范围为37～70℃,最适生长温度为55℃。对菌株进行16SrRNA基因序列分析表明,该菌株为短小芽孢杆菌(Bacillus pumilussp·)。对该菌所产淀粉酶性质的初步研究表明:该酶在80℃具有最高催化活性,在98℃保温30min,仍剩余30%的活力;其最适反应pH为5·0。通过Native-PAGE分析表明菌株Tamy12的粗酶液只含有单一条带的淀粉酶。通过TLC(薄层层析)分析该酶水解淀粉的产物表明,其产物主要为葡萄糖、麦芽糖及3～5个葡萄糖基的寡糖,初步确定菌株所产的淀粉酶为高温α-淀粉酶。Strain Tamy12 was isolated from Tengchong hot spring in Yunnan.The strain can grow at the range of 37 to 70℃,and the optimal growth temperature was 55℃.Physiology characteristics and 16S rRNA gene phylogenetic analysis showed this strain belonged to Bacillus pumilus.A thermostable amylase from this strain was further characterized.The amylase had the maximum activity at 80℃ and pH5.0,respectively,and after incubated at 98℃ for 30min,30% residual activity was detected.Native-PAGE analysis showed that the amylase contained only one type of amylase.TLC analysis demonstrated the amylase could hydrolyze starch and release glucose,maltose,and a range of oligosaccharides,indicating this amylase belonged to thermostable α-amylase.

关 键 词：腾冲热海 短小芽孢杆菌 高温淀粉酶 薄层层析 

分 类 号：Q939.124[生物学—微生物学]