地衣芽孢杆菌对小鼠抗疲劳和免疫调节功能的研究  

Study on anti-fatigue and immunomodulation effects of Bacillus licheniformis on mice

作　　者：高翔[1] 李妍妍[1] 徐彩云[1] 苏秀榕[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《食品工业科技》2010年第9期328-330,共3页Science and Technology of Food Industry

基　　金：浙江省重大科技攻关项目(2006C13089);宁波市科技局资助项目(2008C50027)

摘　　要：目的:为了证明地衣芽孢杆菌(Bacillus licheniformis)能调节肠道微生态并能促使机体产生抗菌活性物质,具有抗疲劳和免疫调节功能,本研究利用小鼠研究了抗疲劳和免疫调节的作用。方法:将小鼠随机分成空白对照组,高、低两个实验组和阳性对照组,灌胃6周后,通过转棒时间、全血乳酸、血清尿素氮(BUN)、肝糖原及肌糖原测定观察小鼠的抗疲劳能力,通过免疫器官相对重量称重、ConA诱导的小鼠脾淋巴细胞转化实验及小鼠碳粒廓清实验研究小鼠的相关免疫学指标。结果:地衣芽孢杆菌能明显延长小鼠转棒时间、降低全血乳酸值和血清尿素氮值、增加肝糖原及肌糖原含量;增强小鼠ConA诱导的脾淋巴细胞转化及小鼠碳粒廓清指数。结论:地衣芽孢杆菌可增强小鼠抗疲劳能力和免疫调节作用。Objective：In order to prove the Bacillus licheniformis that can regulate the intestinal microecology through the effect of biology scrambling oxygen and induce body to produce the antibacterial material,have anti-fatigue and immune regulatory function,the effects of Bacillus licheniformis on anti-fatigue and immunomodulation effect in mice were studied in this article.Methods：Mice were randomly divided into normal group,group of low-dose,group of high-dose and positive group.After 6 weeks,claiming time,blood lactic acid,BUN,hepatic glycogen and muscle glycogen were tested for the anti-fatigue,and the index of spleen and thymus organs,the capacities of lymphocyte proliferation induced by Con A,the clearance rate of carbon particles were determined for the immunomodulation.Results：In comparison with the control group,the Bacillus licheniformis could improve the claiming time,reduce the contents of blood lactic acid and BUN and add the contents of hepatic glycogen and muscle glycogen.The capacities of lymphocyte proliferation induced by Con A and the indexes of the clearance rate of carbon particles were enhanced.Conclusion：Bacillus licheniformis has the ability of anti-fatigue and improving the immunomodulation effect in mice.

关 键 词：地衣芽孢杆菌 抗疲劳 免疫调节 

分 类 号：Q939.124[生物学—微生物学]