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机构地区:[1]上海交通大学医学院附属第九人民医院·口腔医学院儿童预防科,上海市口腔医学重点实验室,上海200011
出 处:《上海口腔医学》2010年第4期423-426,共4页Shanghai Journal of Stomatology
基 金:上海市科学技术委员会科研计划项目(074119644,08DZ2271100);上海市重点学科建设项目(S30206)~~
摘 要:目的:应用数码照相、图像分析及染色技术测定离体牙脱矿及再矿化前后牙色度RGB值的变化。方法:选取无龋健康的上颌乳中切牙36颗,随机分为3组。在所有样本牙唇面中1/3处暴露3mm×4mm的开窗面。用2%亚甲基蓝对样本开窗面染色3min后,数码照相。应用数码图像分析技术测定开窗面牙色度RGB1值;再用32%磷酸凝胶酸蚀开窗面1min后,按上述过程重新染色、拍照,测定牙色度RGB2值;然后将样本置于人工唾液中进行再矿化,按矿化时间平均分为3组(组1、2、3),分别再矿化24h、48h和72h,取出后再染色、拍照、测定RGB3值。应用SNK检验对脱矿及再矿化前后RGB值进行比较分析。结果:组1的RGB1、RGB2、RGB3分别为54.58±12.66、24.32±2.4和29.96±3.32;组2分别为58.71±5.85,25.21±2.84和33.66±4.6;组3分别为60.58±8.10、24.41±2.47和34.59±3.53。3组的RGB1、RGB2、RGB3间两两比较差异均有显著性;3组间的RGB1、RGB2值两两比较差异均无显著性;组1与组2、组3的RGB3值间均有统计学差异,而组2与组3的RGB3无统计学差异。结论:数码照相、图像分析及染色技术相结合,可以识别脱矿及再矿化前后牙色度RGB值的变化,用于脱矿和再矿化的研究。PURPOSE: Digital photography,image analysis and tooth-dying were used to detect the change of tooth color RGB value when the ex vivo teeth were de-or re-mineralized.METHODS: Thirty-six healthy maxillary deciduous incisors were assigned randomly to 3 groups.3mm×4mm window was exposed in the center of the labial surfaces for each sample.The windows were dyed using 2% methylene blue then was photographed.The RGB1 value was measured by image analysis.Then the windows were etched 1 min using 32% phosphoric acid gel.The procedures of tooth-dying,photography repeated in the same way and RGB2 value was measured.The windows were re-mineralized in artificial saliva 24h,48h,72h for group 1,2 and 3 respectively.Then the windows were dyed and photographed again,RGB3 value was measured.The RGB value was analyzed using SNK test.RESULTS: In group 1,the average values for RGB1,RGB2,RGB3 were 54.58±12.66,24.32±2.4,29.96±3.32,respectively.In group 2,they were 58.71±5.85,25.21±2.84,33.66±4.6,respectively.And in group 3,they were 60.58±8.10,24.41±2.47,34.59±3.53,respectively.Within each group,RGB1,RGB2,RGB3 values were statistic different between each other.Between the three groups,RGB1、RGB2 values were not significantly different.The difference of RGB3value was significant between the group 1 and 2,between the group 1 and 3,while the difference between group 2 and 3 was not significant.CONCLUSIONS: Digital photography,image analysis combined with tooth-dying may recognize the change of tooth color RGB value when the ex vivo teeth were de-or remineralized.They may be adopted in teeth de-mineralization and re-mineralization study.
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