电子鼻zNose^(TM)构建棉花挥发物指纹图谱及被害棉花挥发物释放节律分析  

Application of Electronic Nose zNose^(TM) for Construction of Volatiles Fingerprint Library and Analysis of Real Time Release Rhythm of Volatiles from Damaged Cotton

作　　者：潘洪生[1,2] 赵秋剑[2] 赵奎军[1] 张永军[2] 吴孔明[2] 郭予元[2] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193

出　　处：《应用与环境生物学报》2010年第4期468-473,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点基础研究发展规划项目(No.2007CB0902);国家自然科学基金项目(Nos.30871640;30330410);植物病虫害生物学国家重点实验室项目(No.SKL2007SR01)联合资助~~

摘　　要：采用电子鼻zNose^(TM)对棉花植株常见挥发性化合物进行定性分析,确定每类化合物的相对保留指数,构建棉花植株常见挥发性化合物特征谱库.采集棉铃虫幼虫取食诱导棉株挥发性化合物,利用电子鼻构建棉铃虫幼虫危害棉株的指纹图谱.比对健康棉株与棉铃虫幼虫危害棉株的色谱图及指纹图谱发现,棉铃虫幼虫危害棉株的挥发物的种类和含量均比健康棉株的多,而且未去除棉铃虫幼虫的危害处理与去除棉铃虫幼虫的危害处理相比,被诱导挥发物的含量显著升高.通过电子鼻实时监测棉花植株各处理β-蒎烯、β-月桂烯等挥发性化合物的释放节律.结果显示,当棉铃虫幼虫危害棉株2h后,β-蒎烯的释放量明显增加,与对照相比差异显著.β-月桂烯在棉铃虫幼虫危害2h的释放量与对照相比没有显著差异,在棉铃虫幼虫危害24h释放量显著增加.6～8h β-蒎烯和β-月桂烯分别达到各自最大释放量,随后缓慢回落,但仍然保持较高释放水平.By using electronic nose zNoseTM,the common volatile compounds of cotton plant were qualitatively analyzed in laboratory.The relative retention index of each compound was determined,and the cotton common volatiles library was then constructed.In addition,the volatiles from the cotton plant infested by cotton bollworm（CBW）larvae were collected,based on the library,and fingerprint database of the cotton plant damaged by CBW larvae was obtained.Comparing control cotton plant with the infested cotton plant,it was found that both the kinds and contents of the volatiles from the infested cotton plant were notably higher than those of the control.Furthermore,the contents of the volatiles from the cotton plant damaged by CBW larvae with larvae treatment were significantly higher than those of the cotton plant damaged by CBW larvae without larvae treatment.Moreover,by using zNose^（TM） the real time release rhythm of β-pinene and β-myrcene from the infested cotton plant was monitored.The results showed when cotton plant was damaged by CBW larvae for two hours,β-pinene release significantly increased compared with the control.However,there was no difference in the contents of β-myrcene between the cotton plant damaged by CBW for two hours and the control.After invested for 2～4 hours,the β-myrcene content markedly increased.And after 6～8 hours,the contents of β-pinene and β-myrcene reached their maximum release,respectively,and then slow down,but still maintained a high level of release.Fig 3,Tab 1,Ref 24

关 键 词：电子鼻 指纹图谱 棉花 植物挥发物 释放节律 

分 类 号：S562[农业科学—作物学]