复合双歧胶囊对小鼠免疫功能的影响  

Effects of compound capsule containing bifidobacterium and lactobacillus acidophilus on immunity function of mice

作　　者：李立[1] 张新萍[2] 王秋水[3] 张焱[1] 王海玉[1] 陈东方[1] 

机构地区：[1]河南省疾病预防控制中心,郑州450016 [2]鹤壁职业技术学院医学院临床教研室,鹤壁458000 [3]乐山市中心血站,乐山614000

出　　处：《郑州大学学报（医学版）》2010年第4期635-637,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨复合双歧胶囊对小鼠免疫调节作用。方法:将192只BALB/c小鼠随机分为4批,每批均为48只,一批动物进行脏器/体质量比值和小鼠碳廓清实验;一批动物进行绵羊红细胞诱导小鼠足趾增厚(DTH)、抗体生成细胞检测和血清凝血素测定;一批动物进行小鼠腹腔巨噬细胞吞噬鸡红细胞实验;一批动物进行刀豆蛋白A(ConA)诱导的小鼠脾淋巴细胞转化实验和乳酸锂脱氢酶法测定NK细胞活性。每批动物分为低、中和高3个剂量组(0.2、0.4和1.2g/kg)和溶剂对照组,每组12只,分别给予复合双歧胶囊和蒸馏水。结果:高剂量组复合双歧胶囊可提高小鼠碳廓清能力(F=2.872,P=0.047),促进NK细胞活性(F=3.866,P=0.015)和血清凝血素的生成(F=3.748,P=0.018);中剂量和高剂量组复合双歧胶囊能促进ConA诱导的小鼠脾淋巴细胞转化能力(F=8.213,P<0.001)和抗体生成细胞的生成(F=65.033,P<0.001),提高小鼠腹腔巨噬细胞吞噬鸡红细胞能力(吞噬百分率:F=7.500,P<0.001;吞噬指数:F=9.715,P<0.001);3个剂量组均可增强绵羊红细胞诱导小鼠DTH能力(F=11.666,P<0.001)。4组脾脏和胸腺的免疫器官/体质量比值比较,差异无统计学意义(F=1.302和0.762,P均>0.05)。结论:复合双歧胶囊对小鼠的细胞免疫、体液免疫和单核-巨噬细胞功能和NK功能有促进作用,即具有增强免疫力功能。Aim：To observe the immuneregulating effects of the compound capsule containing bifidobacterium and lactobacillus acidophilus on mice.Methods：A total of 192 mice were randomly divided into four groups,and each group was divided into four groups（low dose group,middle dose group,high dose group and control group）.In vivo normal mice,the tests were made including the ratio of spleenthymus/body weight,T lymphocyte proliferation of mouse spleen induced by ConA,the response of delayed hypersenitivity,the amount of hemolytic antibility in serum,antibotyproducing cell assay,phagocytic activity of macrophages and the ability of macrophages for carbon particle clearance and cytotoxicity of NK cells by LDH method.Results：The compound capsule containing bifidobacterium and lactobacillus acidophilus could in-crease the ability of macrophages for carbon clearance（F = 2.872,P = 0.047）,the amount of hemolytic antibility in serum（F =3.748,P =0.018） and the activity of NK cells（F =3.866,P = 0.015） in the high dose group.Compared with the control group,significant differences were observed in the middle and high dose groups in the quantity of antibody-producing cells（F = 65.033,P 0.001）,the ability of T lymphocyte proliferation of mouse spleen induced by ConA（F = 8.213,P 0.001） and phagocytic activity of macrophages（phagocytic rate：F = 7.500,P 0.001;phagocytic index：F = 9.715,P 0.001）.Difference was also observed in the capacity of the response of delayed hypersensitivity induced by SR-BC（F = 11.666,P 0.001） in three dose groups.But significant differences were not observed in the ratio of spleen-thy-mus/body weight（F was 1.302 and 0.762,P 0.05）.Conclusion：The compound capsule containing bifidobacterium and lactobacillus acidophilus may have enhancing effects on cell immumity,humoral immumity,the macrophage engulfining function and NK cell cytotoxicity in mice,so it has the function of enhancing immunity.

关 键 词：双歧杆菌 嗜酸乳杆菌 低聚糖 免疫调节 小鼠 

分 类 号：R115[医药卫生—公共卫生与预防医学]