新型介观流控生物芯片系统及其在β珠蛋白生成障碍性贫血检测方面的应用  

Study on Meso-fluidic Biochip System and Its Application in β-Thalassemia Genotyping

作　　者：霍昊[1] 叶邦策[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《现代检验医学杂志》2010年第4期30-33,共4页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金仪器专项资助项目（No.20627005）.

摘　　要：目的 构建一种新型介观流控微珠生物芯片检测体系,并设计介观流控微珠生物芯片分析仪,应用于β珠蛋白生成障碍性贫血检测.方法 将异硫氰酸化微珠作为寡核苷酸探针载体构建微珠芯片,应用介观流控微珠生物芯片分析仪,通过杂交反应捕获靶标DNA并扫描荧光信号,根据位置寻址得到结果.结果 该系统的检测下限可以达到1×10-10 mol/L,检测时间在30 min以内.利用此体系对人类β珠蛋白生成障碍性贫血的常见突变位点β-28,β-29,β17,HbE26,IVS1-1,β41-42,β43和IVS（II-654）进行了单碱基突变检测,与传统测序结果吻合.结论 新型介观流控芯片分析平台整合度高,可有效应用于基因检测,提高了分析效率,具有广阔的应用前景.Objective To design and establish a novel meso-fluidic biochip system and biochip analyzer. Methods The CyS-labled target samples were introduced into the micro channel and captured by the probes on the NCS-micro beads. Compared to the position of the addressable micro-beads could get the result of geno-analysis. Results The lowest target concentration that could be detected by the mesofluidic analysis system was 1 ×10^-10mol/L,and it only took 30 rain for one test. The system was applied in the genotyping of 8 mutant sites of β-Thalassemia β-28,β-29,β17,HbE26,IVS1-1,β41-42, 643 and IVS（II-654）. Conclusion It is a highly integrated geno-analysis system which has got kinds of priority such as highly sensitive,multiplex analysis,and low pollution.

关 键 词：介观流控 生物芯片 Β珠蛋白生成障碍性贫血 基因突变检测 

分 类 号：Q503[生物学—生物化学] R556.61[医药卫生—血液循环系统疾病]