次氯酸钠衍生-气相色谱-电子捕获检测法测定食品中甜蜜素  

Determination of sodium cyclamate in food by gas chromatography-electron capture detector after derivatization with sodium hypochlorite

作　　者：余胜兵[1] 黄伟雄[1] 吕芬[1] 李少霞[1] 刘锐[1] 朱炳辉[1] 

机构地区：[1]广东省疾病预防控制中心,广州510300

出　　处：《中国卫生检验杂志》2010年第8期1860-1861,共2页Chinese Journal of Health Laboratory Technology

基　　金：广东省医学科研基金(B2010027)

摘　　要：目的:建立食品中甜蜜素的气相色谱检测方法。方法:甜蜜素在酸性条件下与次氯酸钠反应生成电负性较强的N,N-二氯环己胺,经正己烷提取,HP-5柱分离,电子捕获检测器检测,外标法定量。结果:本法在0.10～10.0 mg/L范围内线性关系良好(r=0.9990),检出限为0.025 mg/L(S/N=3)。样品加标回收率为82%～91%,相对标准偏差小于6.1%。结论:本法具有操作简单、快速、灵敏度高等优点,适合于食品中微量甜蜜素的测定。Objective：To establish a method for determination of sodium cyclamate in food by capillary gas chromatography.Methods： Sodium cyclamate was derivatized with sodium hypochlorite to form N,N-dichloridecyclohexylamine under acid condition.N,N-dichloridecyclohexylamine was extracted with hexane and then separated by HP-5 capillary column and detected by electron capture detector（ECD）.The amount of sodium cyclamate was quantified by external standard method.Results： The range of linear range was 0.10～10mg/L（r=0.9990）.The limit of detection was 0.025 mg/L（S/N=3）.The relative standard deviation（RSD） was less than 6.1%（n=5） and the recoveries of the spiked samples were 82%～91%.Conclusion： This method is simple,fast,and sensitive,and is suitable for the determination of low concentration of sodium cyclamate in food.

关 键 词：甜蜜素 食品 气相色谱法 

分 类 号：O657.71[理学—分析化学]