毛细管气相色谱法快速检测食品中脱氢乙酸  

Determination of dehydroacetic acid in food by capillary gas chromatography

作　　者：洪萍[1] 应杏秋[1] 徐陆妹[1] 韩见龙[2] 

机构地区：[1]杭州市萧山区疾病预防控制中心,杭州311201 [2]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2010年第8期1882-1883,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立食品中脱氢乙酸的毛细管气相色谱快速检测方法。方法:样品经脱脂、脱蛋白,加盐酸酸化后,乙酸乙酯提取。采用HP-5毛细管柱程序升温分离,FID检测器检测。标准曲线法定量。结果:脱氢乙酸含量在50μg～1000μg范围内呈线性关系,线性相关系数为0.9999,样品加标回收率为91.4%～100.2%,RSD为0.9%～2.0%,最低检出量为15.0μg。结论:本法简便、快速,可用于食品中脱氢乙酸的快速检测。Objective：To develop a method for the determination of dehydroacetic acid in food by capillary gas chromatography.Methods：The samples were defatted and depoteinized,and added with the hydrochloric acid.Subsequently,ethyl acetate was used to extract dehydroacetic acid,and HP-5 capillary column for separation with FID detector dection.Results：The linear range of the method was 50 μg～1000 μg,the correlation coefficient was 0.9999.The recoveries ranged from 91.4% to 100.2%.With the RSD of 0.9%～2.0%,and the detection limit was 15.0 μg.Conclusion：This method can be applied for the rapid analysis of dehydroacetic acid in food.

关 键 词：食品 脱氢乙酸 快速检测 

分 类 号：O657.71[理学—分析化学]