快速分离液相色谱法同时测定食品中10种脂溶性抗氧化剂  

Simultaneous determination of 10 fat-soluble antioxidants in foods by rapid resolution liquid chromatography

作　　者：李淑娟[1] 冯楠[1] 安娟[1] 蔡会霞[1] 李刚[1] 高旭[1] 刘畅[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123

出　　处：《中国卫生检验杂志》2010年第8期1890-1892,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家质检总局基金资助课题(2007IK263)

摘　　要：目的:建立食品中PG、THBP、BHT、EMQ、NDGA、BHA、OG、Ionox-100、DG和TBHQ 10种脂溶性抗氧化剂的快速分离液相色谱法(RRLC,Rapid Resolution LC)。方法:样品经乙腈提取浓缩后,使用SB-C18液相色谱柱进行液相色谱分析,采用外标法定量。结果:在添加水平范围内,10种抗氧化剂的添加回收率在66.4%～116.3%之间,相对标准偏差4.2%～7.4%。结论:方法准确度和稳定性好,操作快捷,测定下限能够满足限量要求。Objective：To develop a RRLC method for the determination of PG,THBP,BHT,EMQ,NDGA,BHA,OG,Ionox-100,DG and TBHQ in foods.Methods： The fat-soluble antioxidants in samples were extracted with acetonitrile,the separation was performed on a SB-C18 column,and quantified by the external standard curve.Results： The recoveries were 66.4%～116.3%,with relative standard deviations between 4.2% and 7.4%.Conclusion： The method is accurate and suitable for the identification and quantification of fat-soluble antioxidants residue in foods.

关 键 词：RRLC 食品 脂溶性抗氧化剂 测定 

分 类 号：O657.72[理学—分析化学]