汽爆玉米秸秆同步糖化发酵产乙醇的工艺优化  

Process optimization of ethanol production from steam exploded corn stalk by simultaneous saccharification and fermentation

作　　者：赖智乐[1] 常春[1] 马晓建[1] 魏巍[1] 罗永生[1] 王铎[1] 

机构地区：[1]郑州大学化工与能源学院,郑州450001

出　　处：《农业工程学报》2010年第7期250-254,共5页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家科技支撑计划(2007BAD66B04);河南省教育厅自然科学研究计划项目(2008B480004)

摘　　要：该文对同步糖化发酵玉米秸秆生产纤维乙醇的工艺条件进行了研究,分别利用单因素试验和正交试验考察了影响发酵的工艺条件。结果显示:温度37℃、pH值4.8、底物15%(质量分数)、表面活性剂Tween-200.15%(质量分数)、纤维素酶用量30U/g、酵母用2%的葡萄糖溶液活化,接种量5‰(相对底物干质量)、培养基的组成:KH2PO42.5g/L,(NH4)2SO42.0g/L,MgSO4·7H2O0.15g/L,CaCl20.35g/L、发酵时间60h为同步糖化发酵(SSF)生产乙醇的最优工艺条件,该条件下乙醇的体积分数为2.85%,乙醇得率达理论值的88.12%。The process of cellulose ethanol production by simultaneous saccharification and fermentation（SSF） was studied in this paper,and the optimum conditions of the process were found by single-factor experiment and orthogonal test.The conditions were as follows：reaction temperature 37℃,pH value 4.8,loading of substrate 15%,loading of 0.15% Tween-20 as surfactant,loading of cellulose 30 U/g,the yeast active by 2% glucose,yeast inoculums 5‰,and the medium compositions were KH2PO4 2.5 g/L,（NH4）2SO4 2.0 g/L,MgSO4·7H2O 0.15 g/L,CaCl2 0.35 g/L.Under the optimum conditions,the volume of ethanol is 2.85（v/v）,then the ethanol yield can reach to 88.12% of the theoretic results after 60 h.

关 键 词：乙醇 酵母 秸秆 同步糖化发酵 纤维素酶 培养基 

分 类 号：TK6[动力工程及工程热物理—生物能]