超微弱发光用于番茄果实冷害发生程度的无损监测  

Nondestructive Examination of Tomato Chilling Injury by UltraweakLuminescence

作　　者：赵丹莹[1,2] 生吉萍[1,3] 丁洋[1] 申琳[1] 范蓓[3] 刘灿[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京市西城区疾病预防控制中心,北京100011 [3]中国农业科学院农产品加工研究所,北京100931

出　　处：《光谱学与光谱分析》2010年第9期2493-2495,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30571291;30972065);科技部"十一五"科技支撑项目(2006BAD20B07);国家公益性行业科研专项项目(200803033)资助

摘　　要：超微弱发光测定法可快速、灵敏地反映生物体在逆境下的生理状态,已开始得到广泛应用,但有关其在采后园艺产品冷害发生监测中的应用尚未见报道。文章通过分析番茄果实在不同冷害状态下的超微弱发光规律,及其与冷害程度间的关系,探讨超微弱发光测定法在监测采后番茄果实冷害中应用的可能性。结果表明,果实超微弱发光强度随冷害程度的提高而上升,且在表现冷害症状前,冷害果实的超微弱发光值已显著高于正常果实(P<0.01)。不同冷害程度下果实的冷害发病率、冷害指数分别与超微弱发光值的相关系数达到0.901 6和0.957 7(P<0.01),证明超微弱发光可以准确反映番茄果实冷害程度。Ultraweak luminescence（UWL） is an universal phenomenon of photons emission in living organism.It is intrinsically connected with biological processes,and it closely links with biochemical,physiological and pathological conditions in organisms under stress.UWL testing is widely used in clinical medicine,agriculture,environmental protection and food industry etc.But it was not applied in testing physiological disease of agricultural product in storage.This study used tomato fruit as material to find rule of UWL in fruits under chilling stress,which is the main problem in tomato fruit storage. To obtain fruits with different chilling injury degree,before cold storage a group of fruits were treated with cold shock,the effective method to decrease chilling injury,which showed alleviative chilling injury symptom compared with untreated fruits.It was found that UWL rose with the degree of chilling injury,furthermore UWL went up remarkably（P0.01） before symptom of chilling injury happening.Correlation analysis between UWL and chilling injury rate and chilling injury index showed that correlation coefficient was 0.901 6 and 0.957 7（P0.01）respectively.In conclusion,UWL could factually reflect the degree of chilling injury.

关 键 词：超微弱发光 冷害 逆境 检测 番茄 果实 

分 类 号：S641[农业科学—蔬菜学]