变形链球菌GTase高表达株免疫奶牛应用研究  

Production of bovine milk antibody to Streptococcus mutans by immunization of cows

作　　者：边专[1] 樊明文[1] 杜民权[1] 张平[1] 陈文霞[1] 王茜 

机构地区：[1]湖北医科大学口腔医学院,武汉市430079

出　　处：《口腔医学纵横》1999年第1期1-3,共3页Journal of Comprehensive Stomatology

基　　金：国家自然科学基金!编号39570764

摘　　要：目的：观察防龋疫苗免疫奶牛后，牛乳中特异性抗体的产生规律。方法：应用变链菌ＧＴａｓｅ—Ｉ高表达株免疫怀孕奶牛，收集牛乳，经间接ＥＬＩＳＡ检测牛乳中特异性抗变链菌抗体。结果：抗变链菌特异性抗体出现于初乳中，其含量于分娩后 １０ｄ达到高峰，在末次免疫后，特异性抗体可在 ６０ｄ内维持在较高水平。巴氏消毒（６２. ５℃，３０ｍｉｎ）对免疫牛乳中ＩｇＧ活性无明显影响。结论：变链菌ＧＴａｓｅ高产株可诱导奶牛分泌高效价抗变链菌特异ＩｇＧ。Objective: To stimulate anti - S. mutans antibody in bovine milk. Methods: A S. mutans GTase - I overexpression strain was used as a vaccine to immunize pregnant cows. Bovine milk was collected and the specific IgG to S. mutans was evaluated by ELISA. Result: Anti - S. mutans IgG was appeared in colostrum and milk and maintained at a significant level for 60 days after final immunization. The IgG activity was not inactivated by heating at 62. 5℃ for 30 min. Conclusion: Anti - S. mutans antobody in bovine milk is effectively induced in this immunization model.

关 键 词：牛乳 抗体 变形链球菌 GTase高表达株 龋齿 

分 类 号：R780.1[医药卫生—口腔医学] TS252.1[医药卫生—临床医学]