SEDL基因及其编码蛋白sedlin在小鼠多种组织中表达研究  

Expressions of SEDL gene and its encoded protein sedlin in a variety of tissues in mice

作　　者：胡娜[1] 熊丰[1] 廖飞[2] 王旭荣[1] 

机构地区：[1]重庆医科大学附属儿童医院内分泌科,重庆400014 [2]重庆医科大学生物化学与分子药理学重点实验室,重庆400016

出　　处：《重庆医科大学学报》2010年第7期970-973,共4页Journal of Chongqing Medical University

基　　金：重庆市自然科学基金计划重点项目(编号:CSTC;2007BA5028)

摘　　要：目的:研究SEDL基因及其编码蛋白sedlin在小鼠多种组织中的表达。方法:采用Real-TimePCR和Western Blot检测小鼠9种组织中SEDL基因和sedlin蛋白表达水平及1、2、4周龄小鼠骨组织中的SEDL基因及sedlin蛋白表达水平。结果:①所检测小鼠9种组织中均有SEDL基因及对应的sedlin蛋白表达。组织中SEDL基因及sedlin蛋白表达量上有统计学差异(P<0.0001),以骨组织中SEDL基因及sedlin蛋白表达水平高。②不同周龄小鼠骨组织中,SEDL基因及sedlin蛋白的相对表达水平有统计学差异(P<0.01),二者的表达量随年龄变化的趋势相吻合。结论:SEDL基因和sedlin蛋白在所测组织中的表达相一致。二者的表达量在组织间均有差异性,其中以骨组织中表达水平最高。小鼠骨组织中SEDL基因及sedlin蛋白的表达量上有相同时相性改变。Objective：To investigate the expressions of SEDL（X-linked spondyloepiphyseal dysplasiatarda,SEDL）gene and its encoded protein sedlin in a variety of tissues in mice.Methods：Real-Time PCR and Western Blot were used to detect the expression levels of the SEDL mRNA and sedlin protein in 9 tissues in mice,respectively.And the expression levels of them in bone tissues in mice of 1,2 and 4-week-old were also measured in the same way.Results：①Expressions of SEDL gene and its corresponding sedlin protein were existed in 9 tissues detected in mice.The relative expression levels of SEDL mRNA and sedlin protein in tissues in mice were significantly different （P0.0001）,and with a high level in bone.②In bone tissues of age varied mice,the relative expression levels of SEDL gene and sedlin were obviously different（P0.01）,and age-related changes in expressions were identical with each other.Conclusion：It was concluded that expressions of SEDL gene and sedlin were consistent in tested tissues.Expression levels of them were different among tissues and with a high level in bone.In bone tissues of mice,the expressions of SEDL gene and sedlin had the same changes with time.

关 键 词：SEDL基因 Sedlin 脊椎骨骺发育不良 

分 类 号：R394.2[医药卫生—医学遗传学] R681.1[医药卫生—基础医学]