反式C18:1对血管内皮细胞损伤的影响  

EFFECTS OF TRANS C18:1 ON ENDOTHELIAL INJURY

作　　者：邱斌[1] 刘蓉[1] 邓泽元[1] 范亚苇[1] 李静[1] 胡蒋宁[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《营养学报》2010年第4期328-330,335,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30972482);江西省学术带头人计划(No.2008DD00900);教育部博士点基金(No.20070403002);江西省自然科学基金(No.2008GQY0023)

摘　　要：目的通过检测相关细胞损伤指标,探讨trans C18:1对血管内皮细胞的损伤机制。方法体外细胞培养方法将含trans C18:1终浓度分别为0、50、100、200、400、600μmol/L的达氏修正依氏培养基(DMEM)与血管内皮细胞(EC)于37℃、5%CO2共同孵育24～48 h后,分别测乳酸脱氢酶(LDH)活性和一氧化氮(NO)含量及细胞中一氧化氮合酶(NOS)活性,采用单四唑(MTT)法计算trans C18:1对EC作用后细胞存活率的变化。结果 EC的存活率下降。随时间的延长,与trans C18:1的浓度呈剂量依赖关系;NO分泌量和NOS活性的下降及LDH渗出率的升高与trans C18:1浓度呈剂量依赖关系。Trans C18:1的各浓度梯度与对照组比,以上指标都有显著性差异。结论 transC18:1可造成血管内皮损伤。Objective To exoplore the mechanism of endothelial injury through detecting the related injury index.Method Endothelial cells（EC） were cultured with trans C18：1 at different concentrations（0,50,100,200,400,600 μmol/L） for 24-48 h,then nitrogen oxide（NO）,nitrogen oxide synthase（NOS） and lactic acid dehydrogenase（LDH） activities of the culture media and cell lysates were measured spectrophotometrically.The cell viability was measured by monotetarzolium（MTT） after EC cultured with trans C18：1 for 24-48h.Results The decline of cell viability and LDH leakage were dose-dependent on trans C18：1 with time prolongation;The secretory volume of NO and the activity of NOS declined with the rise of trans C18：1 concentration.The series concentration of trans C18：1 compared with the untreated control group had significant difference.Conclusion Trans C18：1 could cause endothelial injury.

关 键 词：TRANS C18:1 内皮细胞 损伤 

分 类 号：R543[医药卫生—心血管疾病]