Bcl-2和P16在膀胱癌的表达与预后的关系  

EXPRESSION OF BCL-2 AND P16 IN TRANSITIONAL CELL CARCINOMA OF URINARY BLADDER AND ITS SIGNIFICANCE

作　　者：冷江涌[1] 张亚正[1] 姚旭东[1] 温凯胜[1] 

机构地区：[1]内蒙古医学院第一附属医院泌尿外科

出　　处：《内蒙古医学院学报》1999年第1期7-11,共5页Acta Academiae Medicinae Neimongol

摘　　要：目的：为探讨ｂｃｌ－２和Ｐ１６基因蛋白在膀胱癌的表达及其临床、病理意义与预后的关系。方法：采用免疫组化技术ＳＡＢＣ法对５１例膀胱癌组织和５例正常膀胱粘膜行原癌基因ｂｃｌ－２和抑癌基因Ｐ１６表达的检测。结果：ｂｃｌ－２在膀胱癌的阳性表达率为８２４％（４２／５１）。在预后中生存组和死亡组之间比较有显著性差异（Ｐ＜００５）。Ｐ１６在膀胱癌的阳性表达率为５１０％（２６／５１）。５例正常膀胱粘膜均阳性，两者比较有显著性差异（Ｐ＜００５）。在病理分级、临床分期和预后的总样本率中比较有显著性差异（Ｐ＜００５）。即随病理分级临床分期的上升和预后的不良，Ｐ１６的阳性表达下降。ｂｃｌ－２阳性而Ｐ１６阴性（２０例）的分布也随病理分级，临床分期的上升和预后不良而上升（Ｐ＜００５）。ｂｃｌ－２和Ｐ１６都阳性（２２例）的分布，随病理分级临床分期升高和预后不良而下降（Ｐ＜００５）。结论：原癌基因ｂｃｌ－２的过度表达和抑癌基因Ｐ１６的缺失突变与膀胱癌的发生发展及预后有密切关系。可作为评估膀胱癌恶性程度及预后的指标。Objective:To study the relationship between the expression of proto-oncogene bcl-2 and MTS1/p16 in transitional cell carcinoma of bladder and prognosis.Methods:Immunohistochemical SABC method was used to detect the expression of proto-oncogene bcl-2 and MTS1/p16 in 51 paraffin-embedded primary transitional cell carcinoma of bladder specimens and 5 normal bladder mucosa specimens.Results:The positive expression of bcl-2 was seen in 82.4%(42/51) tumors.There was statistically significant correlation between prognosis and the positive expression of bcl-2.The positive expression rate in tumors witfh poorer prognosis was higher than that with better prognosis(P<0 05).The expression of P16 protein was positive in 51.0%(26/51) in tumors and negative in all 5 normal bladder mucosae as controls.The statistical significance existed between tumor groups and controls The positive expression rate of p16 descended with increasing grade,stage and poorer prognosis.The clinicopathological characteristics and prognosis were apparently related to the expression of p16(P<0 05).The positive expression of bcl-2 versus negative expression of p16 were seen in 20 of 51 of tumors,with distributing grade Ⅰ 3/11,grade Ⅱ 9/26,grade Ⅲ 8/14,stage Tis-T1 6/28,T2 10/17,T3 4/6,group A 5/20,group B 4/16,and group C 11/15,respectively.Both bcl-2 and p16 were positive expression in 22 of 51 of tumors Their positive expression were higher in tumors with lower grade,stage and better prognosis than that with worse clinicopathological characteristics and poorer prognosis (P<0 05).Conclusion:Overexpression of bcl-2 appeared to be a common event in bladder cancer, that overexpression of the proto-oncogene bcl-2 and inactivation of the MTS1/p16 gene were likely to be contributing factors in development of primary bladder cancer,and that they could be the prognostic indicators of the transitional cell carcinoma of urinary bladder.

关 键 词：膀胱癌 癌基因 遗传学 P16 BCL-2 预后 

分 类 号：R737.140.7[医药卫生—肿瘤]