胃癌组织5q微卫星不稳定性与APC/MCC基因杂合性缺失的研究  

Microsatellite instability of chromosome 5q and loss of heterozygosity at APC/MCC genetic loci in gastric carcinoma

作　　者：王东旭[1,2] 房殿春[1,2] 周晓东 刘为纹[1,2] 

机构地区：[1]解放军第254医院 [2]第三军医大学西南医院

出　　处：《中华消化杂志》1999年第2期107-110,共4页Chinese Journal of Digestion

摘　　要：目的探讨５ｑ微卫星不稳定性（ＭＳＩ）与ＡＰＣ／ＭＣＣ基因杂合缺失（ＬＯＨ）的关系。方法应用ＰＣＲ－ＳＳＬＰ及ＰＣＲ－ＲＦＬＰ技术分析５２例手术切除胃癌组织中ＭＳＩ及ＡＰＣ／ＭＭＣ基因ＬＯＨ。结果５ｑＭＳＩ检出率为３４．０％（１６／４７），ＡＰＣ／ＭＣＣ基因ＬＯＨ率为３１．４％（１１／３５）。早期胃癌５ｑＭＳＩ阳性率为６６．７％（２／３），ＡＰＣ／ＭＣＣＬＯＨ率为５０％（１／２）；进展期分别为３１．８（１４／４４），３０．３％（１０／３３）。两组间无显著差别（Ｐ＞０．０５）。ＭＳＩ及杂合缺失与肿瘤大小、浸润深度、淋巴结转移及临床分期无关。粘液（印戒）细胞癌ＡＰＣ／ＭＣＣＬＯＨ率（５５．６％）显著高于高、中分化管状腺癌（Ｐ＜０．０５）。胃、肠两型胃癌５ｑＭＳＩ及ＡＰＣ／ＭＣＣＬＯＨ差异无显著性及５ｑＭＳＩ与ＡＰＣ／ＭＣＣＬＯＨ无相关性（Ｐ＞０．０５）。结论染色体５ｑＭＳＩ有ＡＰＣ／ＭＣＣ基因ＬＯＨ在两型胃癌的早期发生及发展中起一定作用。染色体５ｑ可能是胃癌的易感部位。Objective To evaluate the role of microsatellite instability (MSI) of chromosome 5q and loss of heterzygosity(LOH) at APC/MCC gene in the development and progeression of gastric carcinoma(GC).Methods MSI of 5q and LOH of APC/MCC were investigated in 52 surgically resected specimens of GC using PCR SSLP and PCR RFLP analysis. Results Positive MSI was found in 16 (34.0%) of 47 successfully amplified cases and LOH of APC/MCC was detected in 11(31.4%) of 35 cancer speciments. The frequencies of MSI and LOH in early stages of GC were 66.7%(2/3) and 50.0%(1/2), respectively, while that in advanced stage of GC, they were 31.8(14/44) and 30.3%(10/33). There was no significant difference between were early and advanced stages MSI and LOH were not correlated to tumor size、invasion depth、lymph node metastasis and clinical staging, LOH in mucoid (Signet ring) cell group(55.6%) was higher than that of well or moderately differentiated group (0.0%)( P <0.05). There was no significant difference of LOH and MSI between intestinal and gastric type of GC and MSI of 5q was not correlated to LOH of APC/MCC gene( P >0.05). Conclusions MSI of 5q and LOH of APC/MCC play imporatent roles in the carcinogensis and progression of both intestinal and gastric types of GC and chromosome 5q may be a susceptible target site of GC.

关 键 词：胃肿瘤 APC/MCC基因 微卫星不稳定性 杂合缺失 

分 类 号：R735.202[医药卫生—肿瘤]