家族性圆柱瘤过表达对槲寄生凝集素诱导HCT116细胞凋亡的影响  

Effects of over-expression of CYLD in apoptosis induced by mistletoe lectin in HCT116 cells

作　　者：林蕾[1] 刘素荣[2] 叶孟[2] 王少敏[2] 倪曙民[2] 

机构地区：[1]宁波大学医学院附属医院皮肤科,浙江宁波315020 [2]宁波大学医学院附属医院肿瘤内科,浙江宁波315020

出　　处：《中华肿瘤防治杂志》2010年第15期1172-1175,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：浙江省自然科学基金(Y205604);宁波市博士基金资助项目(2005A610020);宁波市医学科研项目(200529)

摘　　要：目的:探讨家族性圆柱瘤(CYLD)过表达对槲寄生凝集素诱导人大肠癌HCT116细胞凋亡的影响。方法:MTT法测定细胞的增殖活力,琼脂糖凝胶电泳观察细胞凋亡,流式细胞仪进行细胞周期分析,蛋白质印迹实验和地高辛标记的凝胶电泳阻滞试验(DIG-EMSA)研究细胞凋亡途径。结果:CYLD过表达不影响肠癌HCT116细胞株的生长,但可抑制NF-κB的活性,可增强槲寄生凝集素对HCT116细胞的生长抑制作用,尤其作用12h后CYLD转染细胞细胞的抑制较HCT116细胞和空质粒转染对照细胞更为明显(P<0.01),但不影响槲寄生凝集素对NF-κB的抑制作用,也不影响槲寄生凝集素对IκBα、Bcl-2和Bax的表达。结论:CYLD过表达可抑制NF-κB的活性,并增强槲寄生凝集素对HCT116细胞的生长抑制作用,提示其调控细胞的功能可能取决于NF-κB在细胞中的作用。OBJECTIVE：To investigate the role of over-expression of CYLD in apoptosis induced by mistletoe lectin in human colon cancer HCT116 cells.METHODS：The cell growth inhibiting was measured by the MTT assay;agarose gel eletrophoresis was performed for cell apoptosis;Western blotting was performed for protein expression;DIG-EMSA was conducted to detemine the DNA-binding activation of NF-κB.RESULTS：The over-expression of CYLD had no effects on the growth of HCT116 cells but inhibited the activity of NF-κB.The over-expression of CYLD enhanced the the growth inhibitory effect of mistletoe lectin on HCT116 cells.The inhibitory effect reached the peak at 12 h after mistletoe lectin added into the culture solution （P〈0.01）.The over-expression of CYLD did not affect the inhibition of mistletoe lectin on NF-κB and the expressions of IκBα,Bcl-2 and Bax on HCT116 cells.CONCLUSIONS：The over-expression of CYLD may inhibit the activity of NF-κB and enhance the the growth inhibitory effect of mistletoe lectin on HCT116 cells.The role of NF-κB in the cell may paly an important role in the regulation of cell function of CYLD.

关 键 词：基因 显性 癌 腺样囊性 槲寄生 凝集素类 HCT116细胞系 凋亡 

分 类 号：R739.5[医药卫生—肿瘤]