废液全循环工艺中副产物积累对自絮凝酵母生长和乙醇发酵的影响  

Impact of Accumulated By-products during Distillage Recycling on Growth and Ethanol Fermentation of the Self-flocculating Yeast SPSC01

作　　者：张春明[1] 孜力汗[1] 赵心清[1] 赵大伟[1] 白凤武[1] 

机构地区：[1]大连理工大学生物科学与工程系,辽宁大连116023

出　　处：《过程工程学报》2010年第4期772-776,共5页The Chinese Journal of Process Engineering

摘　　要：在单级搅拌式生物反应器中,以双酶法制备的玉米糖化液为底物,进行了废糟液全循环条件下自絮凝颗粒酵母乙醇连续发酵实验.每隔3d将收集到的发酵液集中精馏处理,得到的废糟液直接用于玉米粉调浆.实验装置连续运行25d.结果表明,乙酸、乳酸、丙酸、糠醛和2-苯乙醇随废糟液循环不断积累,其中丙酸积累最明显,浓度可达2.7g/L,是唯一超过临界抑制浓度的副产物.通过摇瓶添加实验测得上述5种副产物对酵母细胞生长和乙醇发酵的最小抑制浓度分别为3,20,1.5,2和1.5g/L,丙酸与2-苯乙醇及糠醛与2-苯乙醇之间的协同效应最强.Continuous ethanol fermentation coupled with the recycling of waste distillage was carried out in a single agitating bioreactor with the self-flocculating yeast and two-stage enzymatic hydrolysate of corn meal.The fermentation broth collected was distilled to separate ethanol and other volatile products in every 3 d,and the distillage was used to mix corn meal instead of fresh water.The fermentation system was run continuously for 25 d,and the accumulation of acetic acid,lactic acid,propionic acid,furfural and 2-phenyl-ethyl-alcohol,the byproducts that are inhibitory in the growth and ethanol fermentation of the self-flocculating yeast with their minimum inhibition concentrations of 3,20,1.5,2 and 1.5 g/L,respectively,was monitored.It was found that propionic acid with its concentration of 2.7 g/L was the only byproduct accumulated to the level higher than its minimum inhibition concentration.And in the meantime,the synergistic effect of propionic acid and 2-phenyl-ethyl-alcohol as well as furfural and 2-phenyl-ethyl-alcohol were observed.

关 键 词：自絮凝酵母 废液全循环 乙醇连续发酵 副产物抑制效应 

分 类 号：TS262.2[轻工技术与工程—发酵工程]