粘细菌AHB103-1发酵生产抗肿瘤活性物质  

Study on the Production of Antitumor Metabolite by Myxobacteria AHB 103-1

作　　者：徐凤[1] 陶文沂[1,2] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2010年第4期589-594,共6页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2007AA021501)

摘　　要：研究了培养基成分和发酵工艺条件对AHB103-1产生抗肿瘤活性物质的影响。结果表明,较优培养基为:碳源为玉米淀粉;氮源为酵母粉;碳氮比为12∶6;培养基中添加5 mg/L的Gly、Glu和Ala;10 mg/L的Try和Tyr;1.0 mg/L的LiSO4、MnCl2、Na2MoO4和NaH2PO4对抗肿瘤活性物质的生成有明显的促进作用。优化后的发酵工艺条件为:发酵周期7 d,起始pH 7.4,发酵温度30℃;接种种龄4 d;500 mL摇瓶装液量125 mL;摇床转速150 r/min。This study investigated the effect of medium compositions and the environmental conditions on the production of antitumor metabolite and listed as follows： carbon source,corn starch,nitrogen source,yeast extract;the ratio of carbon and nitrogen,12：6;If 5 mg/L Gly,Glu,Ala,10 mg/L Try,Tyr,1.0 mg/L LiSO4、MnCl2、Na2MoO4、NaH2PO4 were supplemented to the culture broth,the yield of antitumor secondary metabolite would be significant improved.The optimum fermentation conditions were as follows： fermentation term,7 d;initiative pH 7.4;fermentation temperature,30 ℃;seed culture term,4 d;the volume of culture liquid contained in 500 mL conical tank,125 mL;rotor speed,150 r/min.

关 键 词：培养基 发酵工艺条件 抗肿瘤活性物质 

分 类 号：TQ460[化学工程—制药化工]