牛奶蛋白质的测定方法优化  

Optimal determination of milk protein

作　　者：魏文平[1] 任丽[1] 王丹慧[1] 

机构地区：[1]内蒙古蒙牛乳业(集团)股份有限公司分析中心,呼和浩特011500

出　　处：《中国乳品工业》2010年第8期41-42,45,共3页China Dairy Industry

摘　　要：国标方法测定牛奶蛋白质检测时间长,利用改进后的凯氏定氮法测定牛奶蛋白质的含量。样品加入硫酸钾、硫酸铜、浓硫酸进行消化,消化后用碱蒸馏,硼酸滴定,通过减少样品的称样量和药品量进行方法优化,优化后消化时间变短,约2 h,且消化后不需要定容即可直接蒸馏滴定。改进后的方法检测结果与国标凯氏定氮法结果无差异,操作简单,省时省力,可快速检测牛奶中蛋白质的质量分数。National standard method of milk protein detection time is long,using the improved Kjeldahl method for the determination of milk protein content.Sample is digested by adding potassium sulfate,copper sulfate,concentrated sulfuric acid,digestion solution is distillated with alkali,titrated with acid,By reducing the weight of sample and drug make methodological optimization,the optimized digestion time becomes shorter,about 2 h,and digestion solution can direct distillation without titration.The improved method results with the GB Kjeldahl method showed no difference,operating simple and saves time and effort to quickly detect the protein content of milk.

关 键 词：蛋白质 牛奶 检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]