大蒜素对人胃癌SGC7901细胞生长的影响  

Effect of allicin on the growth of gastric cancer cell SGC7901

作　　者：刘超侠[1] 孔庆兖[1] 

机构地区：[1]徐州医学院病理生理学教研室,江苏徐州221004

出　　处：《徐州医学院学报》2010年第9期571-573,共3页Acta Academiae Medicinae Xuzhou

基　　金：江苏省教育厅自然科学研究计划(04KJB310143)

摘　　要：目的探讨大蒜素(diallyl trisulfide,allicin)对人胃癌细胞株SGC7901体外生长的影响。方法大蒜素处理胃癌细胞株SGC7901,MTT法检测SGC7901的生长,流式细胞仪检测细胞凋亡率,免疫细胞化学法检测Ki67的表达。结果大蒜素作用于SGC7901细胞后,对其增殖表现出了明显的抑制作用,且呈浓度依赖性;大蒜素作用于SGC7901细胞12、24和48 h后,细胞凋亡率分别为2.41%、7.30%和15.44%,明显高于对照组(P<0.05);大蒜素作用48h后,Ki67的阳性表达率下降(P<0.05)。结论大蒜素可以抑制胃癌SGC7901细胞的增殖和诱导细胞凋亡。Objective To investigate the effects of allicin on the proliferation and apoptosis of gastric cancer cell line SGC7901.Methods The SGC7901 cells were treated by allicin,and MTT assay was used to determine the cell growth.The apoptosis of SGC7901 was detected by flow cytometry（FCM）.The expression of Ki67 in gastric cancer cells was detected by immunohistochemical staining.Results Allicin effectively inhibited the growth of SGC7901 gastric cancer cells in a concentration-dependent manner at a certain range of concentrations.The inhibitory rate was significantly higher than control group.12h,24h and 48h after allicin treatment,the apoptosis rate was 2.41%,7.30% and 15.44%,respectively,with significantly higher effects as compared to the control group（P0.05）.The expression of Ki67 protein was down-regulated by allicin compared with the control group（P0.05）.Conclusion Allicin could inhibit the proliferation and induce apoptosis of gastric cancer SGC7901 cells.

关 键 词：大蒜素 胃癌细胞 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]