甘蔗汁发酵生产丙酮丁醇的研究  

STUDY ON THE ACETONE-BUTANOL FROM SUGARCANE JUICE FERMENTATION

作　　者：彭万峰[1] 熊莲[1] 陈新德[1,2] 袁浩然[1] 危薇[1] 陈勇[3] 

机构地区：[1]中国科学院广州能源研究所,广州510640 [2]中国科学院可再生能源与天然气水合物重点实验室,广州510640 [3]中国科学院广州分院,广州510640

出　　处：《太阳能学报》2010年第8期937-941,共5页Acta Energiae Solaris Sinica

摘　　要：选取8株Clostridium acetobutylicum对甘蔗汁的丙酮丁醇发酵能力进行了研究,筛选出1株丙酮丁醇产量较高的菌株(C.acetobutylicum 810705),比较了添加玉米、木薯和无机盐对该菌株发酵甘蔗汁为丙酮丁醇的影响,对甘蔗汁的发酵条件进行了正交实验,并对将甘蔗汁转化为丙酮丁醇的过程进行了初步研究。结果表明:C.acetobutylicum810705转化甘蔗汁生产丙酮丁醇的能力较好,其最佳发酵条件为:温度37℃,糖质量浓度7.5%,木薯与糖的比例1:1.5,通氧量为微量的氧。丙酮和丁醇的含量分别达到4.6g·L^(-1)和15.4g·L^(-1),丁醇含量比通常发酵的12g·L^(-1)提高了28%,相应的蒸馏能耗将大幅度降低。The abilities of eight Clostridium acetobutylicum strains in conversion of sugarcane juice to acetone-butanol were investigated, and the high acetome-butanol producer, C. acetobutylicum 810705, was selected for further study. The effect of corn, cassava and mineral salt on acetone-butanol fermentation was studied. The orthogonal experiments were carried out for the selection of the optimal fermentation condition. The acetone-butanol fermentation characters of the stain were discussed. The results show that the optimal fermentation condition is temperature of 37 ℃, sugar of 7.5 %, ratio of cassava to sugar 1 ： 1.5, little oxygen. After optimization, the contents of acetone and butanol were 0.46g·L^-1 and 15.4g·L^-1, respectively. The content of butanol has increased by 28% over the general fermentation content 12g·L^-1, and the energy consumption of distillation should be greatly reduced.

关 键 词：丙酮丁醇 甘蔗汁 发酵 木薯 

分 类 号：TQ923[轻工技术与工程—发酵工程]