重组人乳铁蛋白对不同卵巢癌细胞株增殖影响的实验研究  

Study on inhibition effect of recombinant human lactoferrin in vitro on human ovary carcinoma cell lines proliferation

作　　者：仇志琴[1] 虞丰[1] 顾志群[1] 何琳[1] 周晔[1] 蔡蓉[1] 

机构地区：[1]南京医科大学附属无锡第二人民医院妇科,214002

出　　处：《中国妇幼保健》2010年第26期3787-3789,共3页Maternal and Child Health Care of China

基　　金：江苏省卫生厅重点开放项目〔WK0511〕

摘　　要：目的:探讨重组人乳铁蛋白(rhLF)对体外培养的3株人卵巢癌细胞的影响。方法:体外培养SKOV3、HO-8910、HO-8910PM,用MTT法评价rhLF对三者的生长抑制作用。结果:重组人乳铁蛋白对3株细胞均有抑制作用并呈剂量依赖关系,对SKOV3的抑制较另两株显著,不同浓度的rhLF对SKOV3增殖的抑制率达40.95%、62.03%、72.63%,实验对照组细胞无明显的抑制效果。结论:重组人乳铁蛋白可以有效抑制卵巢癌细胞的体外增殖,为其作为新的肿瘤生物治疗剂奠定了理论基础。Objective：To explore the effect of lactoferrin on proliferation of the cultured SKOV3 cells in vitro.Methods：To cultivate SKOV3,HO-8910,HO-8910PM cells in vitro.. MTT assay were used to evaluate the growth inhibition effect of lactoferrin on SKOV3.Results：Lactoferrin significantly inhibited the proliferation of three cells in a dose-dependent manner,the inhibitor rate of SKOV3 was 40.95%,62.03%,72.63% in different concentration,respectively,but had no obvious inhibition on the control cells.Conclusion：Lactoferrin can effectively inhibit the proliferation of Ovary cancer cells,and lay a foundation for exploitation of a new chemistry agent.

关 键 词：乳铁蛋白 卵巢癌 细胞增殖 抑制率 

分 类 号：R737.31[医药卫生—肿瘤]