外阴癌和外阴尖锐湿疣FHIT基因杂合性缺失和微卫星不稳定性  

Study on loss of heterozygosity and Microsatellite instability of FHIT gene in vulva cancer and vulva condyloma acuminatum

作　　者：蒙亚晴[1] 范余娟[1] 杨开选[2] 罗雪慧[1] 徐红[1] 

机构地区：[1]广西医科大学第一附属医院妇产科,广西南宁530021 [2]四川大学华西第二医院病理科

出　　处：《中国妇幼保健》2010年第26期3798-3801,共4页Maternal and Child Health Care of China

基　　金：广西自然科学基金资助项目〔桂科自0640106〕

摘　　要：目的:探讨脆性组氨酸三联体(FHIT)基因杂合性缺失(LOH)和/或微卫星不稳定性(MSI)与外阴癌发生、发展的相关性。方法:应用聚合酶链反应-单链构象多态性分析(PCR-SSCP)法和DNA序列分析方法,分别对30例外阴癌石蜡切片组织中FHIT基因两个微卫星多态性位点D3S1300和D3S1481进行研究,并以40例外阴尖锐湿疣新鲜组织、20例正常外阴组织作对照。结果:D3S1300位点上,外阴癌、外阴尖锐湿疣、正常外阴组织LOH和/或MSI发生频率分别是50.0%(8/16)、22.5%(9/40)、0%,阳性率外阴癌组高于外阴尖锐湿疣组(P<0.05)。D3S1481位点上,外阴癌未提供信息,外阴尖锐湿疣、正常组织的LOH和/或MSI发生频率分别为20.0%、0%(P<0.05)。40例外阴尖锐湿疣中有15例(37.5%)至少存在一个位点的LOH/MSI。结论:①FHIT基因D3S1300位点在外阴癌中具有较高的LOH和/或MSI,FHIT基因频发异常,FHIT基因LOH和/或MSI与外阴癌的发生、发展有关。②外阴尖锐湿疣可能是外阴鳞癌的前期病变。Objective：To study the LOH/MSI on FHIT gene in vulva cancer and vulva condyloma acuminatum.Methods：PCR-SSCP was used to detect the LOH/MSI in 30 cases of vulva cancer,40 cases of vulva condyloma acuminatum and 20 cases of normal vulva epithelium for amplification with 2 polymorphic microsatellite repeats on FHIT gene（chromosome 3p14）.Results：In D3S1300,the LOH/MSI were 50.0%（8/16）in vulva cancer,22.5% in vulva condyloma acuminatum and 0% in normal vulva epithelium.The LOH/MSI in D3S1481 loci could not detected in vulva cancer,20.0% in vulva condyloma acuminatum and 0% in normal vulva epithelium.Conclusion：There are high frequency of LOH/MSI in the D3S1300 and recurrent mutation on FHIT gene.Prompting that：the LOH/MSI on FHIT gene may play an important role in the development and progression of vulva cancer. Vulva condyloma acuminata may be a precancerous stage of vulva cancer.

关 键 词：外阴癌 外阴尖锐湿疣 杂合性缺失 微卫星不稳定性 FHIT基因 

分 类 号：R737.35[医药卫生—肿瘤]