大蒜素诱导胃肿瘤细胞凋亡机制的探讨  

Mechanism of DATS inducing human gastric cancer cell apoptosis

作　　者：夏磊[1] 周玲[1] 崔淑香[1] 牟艳玲[1] 王元书[1] 

机构地区：[1]山东省医学科学院药物研究所,山东济南250062

出　　处：《中华肿瘤防治杂志》2010年第16期1266-1269,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:通过观察大蒜素对人胃癌细胞增殖、凋亡及凋亡过程中Caspases-3和Caspases-8活性的影响,探讨大蒜素诱导凋亡的机制。方法:采用MTT法检测用药后胃肿瘤细胞的增殖;流式细胞仪分析凋亡细胞;用Caspases-3和Caspases-8活性试剂盒检测Caspase变化。结果:大蒜素对胃肿瘤细胞BGC-823、SGC-7901和MKN-28增殖具有明显的抑制作用,72hIC50分别为65.72、83.16和156.23μmol/L;60μmol/L的大蒜素作用24h后,3种胃肿瘤细胞的凋亡率分别为(21.57±0.83)%、(12.31±0.45)%和(10.97±0.54)%,均高于对照组,P<0.01;大蒜素能活化3种胃肿瘤细胞的Caspases-3和活化BGC-823细胞中Caspases-8。结论:大蒜素诱导人胃肿瘤细胞凋亡过程中,Caspases-3的活化起了重要作用。OBJECTIVE：To investigate the changes and significance of Caspases-3 and Caspases-8 activities in diallyl trisulfide （DATS） induced apoptosis of human gastric cancer cells,and explore the apoptosis mechanism promoted by DATS.METHODS：In vitro experiments,MTT assay was used to determine the cell growth inhibition rate.Cell apoptosis analysis was carried out by flow cytometry.The activity of caspase was determined by Caspases-3 and Caspases-8 Colorimetric Assay Kit.RESULTS：Human gastric cancer cell growth was significantly inhibited by DATS.The IC50 of 72 h after treatment by DATS for BGC-823,SGC-7901 and MKN-28 were 65.72,83.16 and 156.23 μmol/L respectively.After treated with 60 μmol/L DATS for 24 h,the apoptosis rates of human gastric cancer cell were （21.57±0.83）%,（12.31±0.45）% and （10.97±0.54）% respectively.The cell apoptosis rates were significantly higher in the experiment group than the control group （P0.01）.DATS activated Caspases-3 of human gastric cancer cells and Caspases-8 of BGC-823 cells.CONCLUSION：DATS induces apoptosis in human gastric cancer cells which may be through the activation of Caspases-3 pathway.

关 键 词：胃肿瘤 大蒜素 细胞凋亡 Caspases-3 Caspases-8 

分 类 号：R735.2[医药卫生—肿瘤]