应用PCR-SSCP非同位素技术检测人宫颈癌nm23-H_1基因突变  

Genomic PCR-SSCP analysis of the mutation of nm23-H 1 gene:a study on cervical carcinoma

作　　者：王忠民[1] 宋梅 

机构地区：[1]大连市妇产医院 [2]新世纪整形康复医院

出　　处：《大连医科大学学报》1999年第2期101-104,共4页Journal of Dalian Medical University

摘　　要：恶性肿瘤病人死亡的主要原因非原发灶本身，而是肿瘤细胞发生转移、浸润全身各器官引起器官功能衰竭所致。宫颈癌是妇科常见的恶性肿瘤，其发病率高居妇科各种肿瘤之首。本研究利用ＰＣＲ－ＳＳＣＰ非同位素技术检测人宫颈癌组织中是否存在转移抑制基因ｎｍ２３－Ｈ１基因突变。其中正常组织２０例，慢性宫颈炎组织３０例均未发现ｎｍ２３－Ｈ１基因突变。２４例宫颈癌组织中１例Ⅲ期低分化宫颈腺癌组织中发生ｎｍ２３－Ｈ１基因突变。结果提示宫颈癌中存在ｎｍ２３－Ｈ１基因突变。Instead of the primary lesion of tumors,the major cause of the death for the patient with tumor is distant metastasis.A safe and simple method,polymerase chain reaction(PCR)-single strand conformational polymorphism(SSCP) analysis,was performed in our study to examine whether there was mutation of tumor suppressor gene,nm23-H 1 gene,in samples from human cervical carcinoma.None mutation was found in 20 normal tissue and 30 chronic cervic it is,while in 24 cervical carcinomas,mutation of nm23-H 1 gene was found in one case in phase Ⅲ.Our results indicates that mutation of nm23-H 1 gene might exist in cervical carcinoma.

关 键 词：单链构象多态性 宫颈癌 基因突变 PCR NM23-H1 

分 类 号：R737.330.2[医药卫生—肿瘤]