变色栓菌产漆酶培养条件及热激对发酵的影响  

Research on the Conditions for Laccase Production from Trametes versicolor and Effect of Heat Shock on Laccas

作　　者：张田[1] 王锋[2] 魏涛[1] 刘春朝[2] 

机构地区：[1]北京联合大学应用文理学院,北京100191 [2]中国科学院过程工程研究所,北京100190

出　　处：《安徽农业科学》2010年第21期11194-11195,11221,共3页Journal of Anhui Agricultural Sciences

基　　金：北京市属高等学校人才强教计划资助项目

摘　　要：[目的]为提高漆酶发酵水平提供一种有效的控制策略。[方法]采用栓菌菌株14001,对其发酵产漆酶条件及热激对漆酶酶活的影响进行研究。[结果]栓菌产酶最适Cu2+浓度为20mg/L,最适起始pH值为4.5,最适接种量为10%,最适培养温度为25℃,并对发酵过程进行pH控制,其酶活比不进行pH控制的酶活最大能提高30%;热激作用能诱导漆酶相应的mRNA产生,提高漆酶酶活1倍以上。[结论]优化后的培养条件和热激均能提高漆酶的发酵水平。[Objective] The study aimed to provide an effective control strategy for enhanceing the level of laccase fermentation.[Method] With strain of trametes versicolor 14001,conditions for laccase production and the effects of heat-shock activity on fermentation was studied.[Result] The results indicated that the optimal fermentation conditions were as follows：Cu2＋ concentration 20 mg/L,initial pH4.5 of the medium,inoculum percentage 10% and at 25 ℃.Enzyme activity improved 30% compared with not pH cortrol.Heat shock could induce the corresponding the mRNA of laccase,increased the laccase by one times.[Conclusion]The optimized performance and heat shock could enhance fermentation leverl of laccase.

关 键 词：热激 漆酶 发酵 

分 类 号：Q814[生物学—生物工程]