喉癌中P53基因突变与突变型P53基因蛋白表达的相互关系及意义  

THE SIGNIFICANCE AND RELATIONSHIP BETWEEN P53 GENE MUTATION AND EXPRESSION OF MUTANT TYPE P53 PROTEIN IN LARYNGEAL CARCINOMAS

作　　者：刘克杰[1,2,3] 王惠忠 张信行[1,2,3] 张广茹 李莉 

机构地区：[1]临沂医学专科学校 [2]淄博侨联医院 [3]临沂市中医医院

出　　处：《滨州医学院学报》1999年第2期111-113,共3页Journal of Binzhou Medical University

摘　　要：目的：探讨喉癌Ｐ５３基因突变与突变性Ｐ５３基因蛋白表达之间的相互关系及意义。方法：对４０例喉癌新鲜手术标本采用ＰＣＲ—ＳＳＣＰ检测Ｐ５３基因突变，免疫组化方法（ＬＳＡＢ）检测突变型Ｐ５３基因蛋白表达，并比较其相互间的关系。结果：４０例喉癌中有２６例基因突变阳性（６５％），Ｐ５３基因蛋白表达阳性者２３例（５７．５％）；Ｐ５３基因突变与其突变型蛋白表达比较，两者皆阳性为４５％，两者均阴性为２２．５％，其一致率为６７．５％（Ｐ＜０．０５）。结论：Ｐ５３基因突变与其蛋白表达相吻合。两种检测方法在喉癌的分子病理学诊断及其预后方面同样具有重要的价值。Objective:To analyse the significance and relationship between p53 gene mutation and the expression of mutant type P53 gene protein in the laryngeal carcinomas.Methods:The fresh tissues of 40 cases of laryngeal carcinomas were used.The mutation of P53 gene was detected by PCR-SSCP and mutant type P53 gene protein was detected by immunohistochemistry staining LSAB method.The results were compared. Results:Out of 40 cases of laryngeal carcinemas,26(65%) showed P53 gene mutation,and 23(57 5%) had positive P53 staining.Comparing the results of PCR SSCP and the staining,both the positive rate was 45% and the negative rate was 22 5%.The concordant rate was 67 5% Conclusion:P53 gene mutation is concordant with protein P53,and P53 immunostaining and SSCP could be used as same as important methods relating to the tumor stage,histopathologic grades and prognosis of laryngeal carcinoma.

关 键 词：喉癌 基因 P53 基因突变 基因表达 

分 类 号：R739.650.2[医药卫生—肿瘤]