防风中色原酮类化合物的抗氧化活性研究  

Anti-oxidant Activities of Chromones from Saposhnikovia divaricata

作　　者：李丽[1] 桂语歌[1] 时东方[1] 刘春明[1] 

机构地区：[1]长春师范学院,吉林长春130032

出　　处：《时珍国医国药》2010年第9期2135-2137,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30873364);吉林省科技厅项目(No.20071102;200905109);吉林省教育厅项目(No.2008-167;2008-170)

摘　　要：目的采用超声提取法进行提取,考察了不同提取时间,不同提取温度及不同提取溶剂对防风中色原酮类化合物提取率的影响。方法采用Folin-Ciocalteu方法测定防风不同提取物总酚含量,采用DPPH抗氧化活性体外评价体系对防风不同提取物的抗氧化活性进行了系统研究。结果超声提取90min样品总酚含量最高,而超声120min乙醇提取物表现出强抗氧化活性。结论防风不同提取物表现出不同的抗氧化活性,但其抗氧化活性与总酚含量没有必然联系。Objective To study the antioxidant activity respectively with different ultrasound-assisted extraction,such as different time,temperature and solvent.MethodsFolin-Cilcaileu method was developed for evaluation of the total phenolic contents（TPCs）of different extracts.The free radical 2,2-diphenyl-1-picrylhydrazyl（DPPH）assay was then used to measure the antioxidant activity of different extracts.ResultsThe TPCs of 80% ethanol extract at 90 min showed the highest TPCs,however,the 80% ethanol extract at 120 min exhibited strong antioxidant activity in DPPH assay.ConclusionThe antioxidant activity in different samples didn＇t show any good correlation with total phenolic contents.

关 键 词：防风 色原酮类化合物 总多酚 抗氧化活性 二苯代苦味肼基自由基 

分 类 号：R285.5[医药卫生—中药学]