K562及K562/VCR对长春新碱诱导凋亡的敏感性  

Sensitivity to Vincristine Induced Apoptosis in K562 and K562/VCR

作　　者：朱玉胜[1,2,3] 吕元[1,2,3] 林果为[1,2,3] 杨正洪 吕沅冈[1,2,3] 张荣兴 胡晓武[1,2,3] 

机构地区：[1]上海医科大学华山医院 [2]中国科学院细胞库 [3]安徽省马鞍山市人民医院检验科

出　　处：《上海医科大学学报》1999年第3期191-193,共3页Journal of Fudan University(Medical Science)

摘　　要：研究人类白血病细胞株Ｋ５６２及耐长春新碱的Ｋ５６２变异株Ｋ５６２／ＶＣＲ对长春新碱诱导凋亡的敏感性的差异，以探讨抗凋亡在白血病多药耐药性（ＭＤＲ）中的作用。方法将Ｋ５６２及Ｋ５６２／ＶＣＲ分别用浓度为０、０．００１、０．０１０μｇ／ｍｌ的长春新碱诱导２４ｈ，用流式细胞仪ＰＩ染色法、ＴＵＮＥＬ法及ＥＬＩＳＡ法分别进行细胞凋亡检测。结果经浓度为０、０．００１μｇ／ｍｌ长春新碱诱导后，Ｋ５６２细胞凋亡率分别为（４．１０±０．１７）％、（１３．３０±３．７４）％，而Ｋ５６２／ＶＣＲ细胞凋亡率分别为（３．６１±０．６９）％、（９．０６±４．２４）％，统计学显示两者差异无显著性（ｎ＝３，Ｐ＞０．０５）。而长春新碱浓度达０．０１０μｇ／ｍｌ时，Ｋ５６２及Ｋ５６２／ＶＣＲ细胞凋亡率分别为（３６．４８±６．７０）％、（１０．４５±３．７１）％，两者差异有极显著意义（ｎ＝３，Ｐ＜０．０１）。ＴＵＮＥＬ及ＥＬＩＳＡ检测结果与此类似。结论Ｋ５６２及Ｋ５６２／ＶＣＲ对长春新碱诱导细胞凋亡的敏感性不同，耐药细胞对药物诱导细胞凋亡的抵抗性增强可能在白血病ＭＤＲ发生中有重要作用。Purpose To study the sensitivity of human leukemic cell line K562 and vincristine resistant cell variant K562/VCR to vincristin induced apoptosis and explore the effect of antiapoptosis on the multidrug resistance of leukemia. Methods K562 and K562VCR cells were treated with vincristine for 24 hours at the concentration of 0,0.001,0.010 μg/ml respectively.Apoptosis was detected with flow cytometry(FCM),TdT-media ted dUTP nick end labeling(TUNEL),and ELISA. Results After the treatment without vincristine,the apoptotic rate of K562 and K562/VCR was (4.10±0.17)% and (3.61±0.69)% respectively and at the concentration of 0.001 μg/ml,the rate was (13.30±3.74)% and (9.06±4.24)% respectively,as determined by FCM.Statistical analysis showed that there was no significant difference in apoptotic rate between K562 and K562/VCR at the above concentrations( n =3, P >0.05).But as the concentration of vincristine was increased to 0.010 μg/ml,the apoptotic rate of K562(36.48±6.70)% was significantly higher than that of K562/VCR(10.45±3.71)% ( n=3,P< 0.01).Similar results were also obtained with TUNEL and ELISA. Conclusions K562 and K562/VCR cells have defferent sensitivity to vincristine induced apoptosis.The resistance of drug resistant leukemic cells to drug induced apoptosis may play an important role in multidrug resistance of leukemia.

关 键 词：白血病 多药耐药性 长春新碱 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]