薯蓣皂苷元微胶囊的制备及体外抗肿瘤活性  

Preparation and in vitro Antitumor Activity of Diosgenin-loaded Microcapsules

作　　者：李彰鑫[1,2] 黄文[3] 薛安[2] 李怡[3] 舒丹[3] 

机构地区：[1]北京大学深圳研究生院城市人居环境科学与技术重点实验室,广东深圳518055 [2]北京大学环境工程系水沙科学教育部重点实验室,北京100871 [3]四川大学生物治疗国家重点实验室,四川成都610041

出　　处：《安徽农业科学》2010年第25期13807-13809,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"十一五"科技支撑项目(2006BAB04A14);四川省科技支撑项目(2008SZ0024)

摘　　要：[目的]制备薯蓣皂苷元微胶囊并对其性能进行表征,探讨微胶囊的体外抗肿瘤活性。[方法]通过喷雾干燥技术制备薯蓣皂苷元微胶囊,采用MTT法对它们的体外抗肿瘤活性进行评价。[结果]以明胶为壁材制备了薯蓣皂苷元微胶囊,并对微胶囊的粒径分布、超微结构和包封率进行了表征。以明胶薯蓣皂苷元配比为5:1制备的微胶囊,包埋率为78%～94%,平均粒径约13μm。细胞试验表明,当药物浓度为40mg/L时,生物体外培养的人结肠癌细胞(C26)有72%被抑制,具有良好的抑癌效果。薯蓣皂苷元微胶囊对C26细胞体外抗增值活性与薯蓣皂苷元相比有了明显提高。[结论]薯蓣皂苷元微胶囊的抗肿瘤活性较薯蓣皂苷元明显提高。[Objective]The paper aims to prepare and characterize diosgenin-loaded microcapsules in order to investigate their in vitro antitumor activity.[Method]The diosgenin-loaded microcapsules were prepared by spray drying technique.Their antitumor activity in vitro was evaluated by MTT method.[Result]By using gelatin as wall materials,the diosgenin-loaded microcapsules were prepared by spray-drying technique,and the particle size distribution,encapsulation efficiency,ultrastructure and other properties of the microcapsules are characterized.The preparation method chosen resulted in diosgenin encapsulation efficiency of 78%-94% and mean particle size of 13 μm.[Result]When diosgenin concentration was 40 mg/L,C26 cells were suppressed to 72%,a good suppression effect will be achieved.Their in vitro antitumor activity in C26 cell was improved compared to that of diosgenin.[Conclusion]The antitumor activity of diosgenin-loaded microcapsules was improved significantly.

关 键 词：薯蓣皂苷元 喷雾干燥 微胶囊 性能表征 抗肿瘤活性 

分 类 号：S56[农业科学—作物学]