基于表位预测的花生过敏原Ara h6免疫交叉反应性研究  

Immunologic Cross-reactivity of Peanut Allergen Ara h 6 on the Basis of Epitope Prediction

作　　者：朱盼[1,2] 陈红兵[1,2] 胡纯秋[1,2] 李欣[1,2] 罗春萍[1,2,3] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学中德联合研究院,江西南昌330047 [3]南昌大学环境与化学工程学院,江西南昌330047

出　　处：《食品科学》2010年第17期318-322,共5页Food Science

基　　金：南昌大学食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200807);江西省主要学科学术和技术带头人培养项目([2004]234号);教育部新世纪优秀人才支持计划项目(NCET-08-07-04)

摘　　要：为了探讨花生过敏原Arah6与其他同源蛋白之间的交叉反应,通过各种生物信息数据库(Genbank,DiscoTope)以及网络工具及软件(BLAST,NPS@和Protean,Clustalx,PyMOL),开展基于表位预测的Arah6交叉免疫反应性研究。结果表明:肽段AA10～15、45～48、53～60、116～118区域可能是Arah6的线性表位优势区域;Arah6的构象型表位优势区有4个,它们存在的区域为AA1～13、35～51、53～59、89～105;基于线性表位预测的15种蛋白质与Arah6可能具有交叉反应性,其中4种(Arai6,conglutin,Arad6和conglutin8)发生交叉反应的概率为100%;基于构象型表位预测的17种蛋白质与Arah6可能具有交叉反应性,其中4种(Arai6,conglutin,Arad6和conglutin8)发生交叉反应的概率为100%。本结果对进一步了解Arah6的过敏性以及指导开展食品中Arah6的免疫学检测具有重要作用。In order to explain cross-reactivity between peanut allergen Ara h 6 and other homologous proteins,various kinds of bioinformationcal databases,including Genbank and DiscoTope,and net tools/software,including BLAST,NPS@,Protean,Clustal x and PyMOL,were used for the epitope analysis between these proteins.It was indicated that the linear epitopes on Ara h 6 existed with high probability in the regions of AA10-15,45-48,53-60 and 116-118,and the conformational epitopes in the regions of AA1-13,35-51,53-59 and 89-105.According to the prediction of linear epitopes,there were fifteen proteins might cross-react with Ara h 6,and the possibility of cross-reactivity of four of them （Ara i 6,conglutin,Ara d 6 and conglutin 8） was 100%.However,according to the prediction of conformational epitopes,the number of proteins could cross-react with Ara h 6 with high probability was 17,and the possibility of cross-reactivity of four of them （Ara i 6,conglutin,Ara d 6 and conglutin 8） was 100%.These investigations will be beneficial for understanding peanut Ara h 6 allergy and help to develop an approach for the immunologic detection of Ara h 6 in foods in the future.

关 键 词：Arah6 花生过敏 表位 交叉反应 预测 

分 类 号：Q517[生物学—生物化学]