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出 处:《复旦学报(医学版)》2010年第5期519-525,共7页Fudan University Journal of Medical Sciences
基 金:国家自然科学基金项目(30840031;30970726)
摘 要:目的分析表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)、Ni2+及其相互作用对人牙龈成纤维细胞(human gingival fibroblast cells,HGF)的生物学效应。方法用MTT法检测HGF的存活率,运用彗星电泳检测细胞的DNA损伤情况,使用流式细胞仪法检测HGF周期变化。结果 Ni2+可呈时间依赖性抑制HGF的生长,造成细胞DNA损伤,并且可使细胞周期阻滞于S期,诱导细胞凋亡。高浓度的EGCG(>200μmol/L)可明显抑制HGF生长,使细胞的DNA损伤。Ni2+与EGCG相互作用后增加了对HGF的抑制(P<0.01),DNA的损伤及凋亡细胞也明显增加(P<0.05)。结论 Ni2+和EGCG相互作用后使Ni2+对HGF的抑制作用增强,加剧HGF的DNA损伤和细胞周期阻滞,并明显增加细胞凋亡。Objective To determine the biological effects of epigallocatechin gallate(EGCG),Ni2+ and their interactions on human gingival fibroblast(HGF) cells. Methods The viability of cells was determined by the MTT reduction assay.To measure DNA damage,we followed a modified version of comet assay.Cell cycle was analyzed by flow cytometry. Results Ni2+ significantly inhibited the growth of HGF cells.Concentrations of Ni2+ greater than 100 μmol/L significantly increased DNA strand breaks damage and arrested the cell cycle.The high concentrations of EGCG(greater than 200 μmol/L) could inhibit the growth of HGF cells.Greater than 150 μmol/L EGCG could increase the DNA strand break damage.The co-treatment of Ni2+ and EGCG could increase Ni2+-induced inhibit effect on HGF cells(P0.01),and the DNA damage was significantly increased(P0.01).Conclusions Ni2+ mixed with EGCG could increase the Ni2+-induced damage on the HGF cells and the blockage of cell cycle.
关 键 词:NI2+ 表没食子儿茶素没食子酸酯 生物学效应 彗星电泳 人牙龈成纤维细胞
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