不同醉酒度浓香型白酒对小鼠血中乙醇代谢的影响  

METABOLIC CHANGES OF MICE ETHANOL AND ACETALDEHYDE CONCENTRATION IN BLOOD AFTER DRUNK BY LIQUOR WITH DIFFERENT DRUNKEN DEGREE

作　　者：郑露[1] 张梦妍[1] 彭彦卿[1] 党好[1] 齐亚娜[1] 许欣[1] 王晶[1] 饶家权[2] 马斌[2] 杜礼泉[2] 

机构地区：[1]四川大学公共卫生学院,成都610041 [2]四川省绵阳市丰谷酒业有限责任公司

出　　处：《现代预防医学》2010年第19期3626-3628,共3页Modern Preventive Medicine

基　　金：四川省科技厅资助项目(2009SZ0101)

摘　　要：[目的]通过检测小鼠灌胃后全血中乙醇、乙醛的代谢变化,为醉酒度评价动物模型感官指标提供佐证与补充。探索能够检测区分不同酒样醉酒度的方法及评价指标。[方法]以48.0%乙醇溶液灌胃作为对照组,观察记录两个品种浓香型白酒对小鼠醉酒时间的影响,计算醉酒度百分比;105只BALB/c小鼠随机分为3组,即48.0%乙醇溶液对照组,酒样1组,酒样2组,以10ml/kg剂量灌胃。于0.5h,1h,1.5h,2h,3h股动脉取血,气相色谱检测血中乙醇乙醛浓度。观察记录两个不同类别浓香型白酒组对小鼠醉酒时间和酒醒时间的影响,比较醉酒时间的差异,计算醉酒度百分比。[结果]酒洋1灌胃后血中乙醇浓度比酒样2高,与感官指标一致,乙醛浓度差异无统计学意义。比较观察指标,两个酒样醉酒时间差异有统计学意义(P﹤0.01),醉酒度百分比分别为161.3%和283.1%。48.0%乙醇对照组醉酒最快。[结论]血中乙醇浓度是影响小鼠醉酒的主要因素,乙醛浓度在不同的酒样间差异没有显著性。醉酒度低的白酒血中乙醇代谢较快,乙醇浓度较低。与48.0%乙醇相比,经过加工发酵的这两个酒样延缓了醉酒时间。本课题建立的方法,可以鉴别区分酒精度相同但醉酒度不同的微小差别的酒样。[Objective] By measuring the metabolic changes of ethanol and acetaldehyde concentration in the mice blood after intragastric infusion by different white spirit,to establish an animal model of alcohol intoxication,alcoholic liver and brain injury.And moreover,to explore the methods which distinguish the degree of drunkenness of wine and evaluation indices. [Methods] 105 BALB /c mice were randomly divided into three groups,48.0% alcohol control group,sample one group,sample two group. They were all infused with 10mg /L dose.At the 0.5,1,1.5,2,3 hours after intragastric infusion,group 1 and group 2 were taken femoral artery blood. And then blood alcohol and acetaldehyde concentration were measured by gas chromatography. More over,the degree of drunkenness was determined by comparing the difference of the ebriety time and sober-up time of the mice. [Results] Blood alcohol concentration of group 1 was higher than group 2,the difference in acetaldehyde concentration was not statistically significant. The result was consistent with oranoleptic indicator. The drunkenness firstly happened in control group. [Conclusion] Blood ethanol concentration is the main factor in mice＇s drunkenness. Acetaldehyde concentration is not statistically significant in different samples. BLood ethanol in the mice with lower drunken degree metabolizes faster,and its concentration is lower. Compared with 48.0% alcohol,the two white spirit samples after zymogene processing postpone the time of beginning drunken. The method established in this study can be used to distinguish white spirits which have the same percent of alcohol but different drunken degrees.

关 键 词：BALB/C小鼠 醉酒 乙醇 乙醛 模型 筛选 

分 类 号：R-332[医药卫生]