三菌混合固态发酵苹果渣产复合酶的研究  

Three bacteria mixed solid-state fermentation of apple pomace enzyme production

作　　者：姜心[1] 陈伟[1] 周波[2] 黄坤勇 

机构地区：[1]山东农业大学食品科学与工程学院,山东省泰安市271018 [2]山东农业大学生命科学学院 [3]天津牧光生物技术有限公司

出　　处：《饲料工业》2010年第18期20-25,共6页Feed Industry

基　　金：国家科技基础条件平台微生物菌种资源整理整合项目(项目编号:2005DKA21201-13);国家科技支撑计划黄淮平原区农田秸秆资源综合利用技术集成研究与示范(2007BAD89B09-16)的资助

摘　　要：利用实验室保藏的多株菌种,对苹果渣进行三元混菌固态发酵产复合酶。以纤维素酶、木聚糖酶、果胶酶活性大小为测定指标,对筛选出单菌活性较高的菌株,按照1:1:1进行3种菌混合发酵,确定混菌后效果较好的3种菌。考虑到试验因素之间的交互作用,以3种菌的接种比例及接种时间间隔作为试验因素,通过正交表交互作用设计试验及分析,确定3种菌混合活性最佳时间间隔为菌种#1、#2、#3同时接种;接种#1体积1ml、接种#2体积2ml、接种#3体积1ml。混菌后酶活性比3种单菌的酶活分别提高了10.9%、2%、20%,达到了优化的目的。We use solid-state fermentation in apple pomaee with multiple strains of bacteria preserved in laboratory to produce enzyme. Cellulase, xylanase, pectinase activity are used for the determination of the indicators. We can selecte activity whose is higher in single bacteria strains, according to 1：1 ： 1 for three bacteria mixed fermentation to determine the effect of the better post-mlxed bacteria three bacteria. Taking into account the interaction between experimental factors, to three kinds of bacteria inoculation ratio and time interval as the experimental factors, through the interaction of orthogonal experimental design and analysis, the best three bacteria mixed-activity time interval strains is # 1 , # 2, # 3 at the same time ;inoculation volume of # 1 is 1 ml;# 2 volume is 2 ml; # 3 volume is 1 ml. Mixed bacterial activity after a single bacteria than the three kinds of activity were increased by 10.9%, 2%, 20%, achieved the purpose of optimization.

关 键 词：三菌混合 复合酶 正交试验交互作用 

分 类 号：S816.6[农业科学—饲料科学]