新农抗702产生菌固体斜面培养基及培养条件优化  

Optimization of solid slant medium and cultural conditions for the New Ag-antibiotic 702 strain

作　　者：阮彩彪[1] 何建[2] 涂晓嵘[1] 李江怀[1] 桑志高[1] 涂国全[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045 [2]南昌市发酵应用技术重点实验室,江西南昌330045

出　　处：《中国酿造》2010年第10期32-35,共4页China Brewing

基　　金：江西省科技支撑计划项目(2009BNA07200)

摘　　要：为了探索新农抗702产生菌链霉菌702产孢子的最佳工艺,确定培养基优化组分与最适培养条件,通过比较6种培养基斜面产孢子情况,选择最佳培养基。通过单因素试验正交试验优化培养条件,得到新抗702产生菌固体斜面产孢子最佳培养基为PDA培养基,蔗糖浓度3%。蛋白胨和氯化钠对其均有抑制作用。最佳培养条件组合为培养温度29℃、初始pH值8、培养时间5d。在此条件下,每支试管斜面产孢子量达到4.42×108个,提高了64.9%。The optimal nutritional and environmental of New Ag-antibiotic 702 strain Streptomyces 702 were investigated by single factors experiment and orthogonal experiment in this paper. The optimal spore-producing medium was PDA medium. The sucrose concentration was 3%. Peptone and sodium chloride has inhibitory effect on strain growth. The optimal culture conditions were as followed： culture temperature 29 ℃, initial pH 8 and fermentation time 5 d. Under these condition, the spore content in a test-tube was 4.42×10^8, which was increased by 64.9% compared to that before optimization.

关 键 词：新农抗702 链霉菌702 孢子 优化 

分 类 号：Q93-335[生物学—微生物学]