包埋-交联结合法固定β-呋喃果糖苷酶的研究  

Immobilization of β-fructofuranosidase by embedding-crosslinking

作　　者：杨秀芳[1] 陈梅[1] 马养民[1] 

机构地区：[1]教育部轻化工助剂化学与技术重点实验室陕西科技大学,陕西西安710021

出　　处：《中国酿造》2010年第10期56-59,共4页China Brewing

基　　金：陕西省教育厅基金项目(08JK238)

摘　　要：分别以海藻酸钠、壳聚糖为载体,采用包埋-交联法固定β-呋喃果糖苷酶。对固定化过程中氯化钙浓度、戊二醛浓度、加酶量、包埋时间、交联时间等因素进行考察;采用正交试验设计对载体制备与酶固定化中的主要条件进行优化。通过对固定化酶活力回收比较,壳聚糖固定化酶活力回收率优于海藻酸钠。β-fluctofuranosidase was co-immobilized by embedding-crosslinking with sodium alginate and chitosan. Some factors affecting the activity of immobilized β-fructofuranosidase were studied including the concentration of calcium chloride, the content of glutaraldehyde, the level of β-fructofuranosidase, embedding time, crosslinking time and so on. The optimal conditions were determined by orthogonal experiments. The results indicated that ehitosan was better than sodium alginate by comparing the yield of immobilized enzyme

关 键 词：壳聚糖 海藻酸钠 固定化 包埋-交联 

分 类 号：TS261.4[轻工技术与工程—发酵工程]