响应面法优化酶解蒸汽爆破玉米芯产木糖  

Response Surface Methodology for Xylose Production from Corncob Pretreated by Steam Explosion

作　　者：王东美[1] 刘桂艳[1] 李春[1] 戴大章[1] 

机构地区：[1]北京理工大学生命学院,北京100081

出　　处：《林产化学与工业》2010年第5期76-80,共5页Chemistry and Industry of Forest Products

基　　金：国家高技术研究发展计划(863计划)(2009AA02Z202)

摘　　要：采用酶法降解经蒸汽爆破的玉米芯半纤维素产木糖,以响应面法对酶解过程进行优化。根据单因素试验结果,选择对木糖产率有显著影响的5个因素,运用Box-Benhnken中心组合原理设计了53全因子试验,确定了酶解蒸汽爆破玉米芯的最佳工艺条件:加酶量13.05 U/g(以玉米芯计)、水料比13.2∶1(mL∶g)、酶解时间5 h、酶解温度47.3℃、pH值5.84,在此优化条件下,木糖产率高达83.24%。Corncob pretreated by steam explosion was used as the substrate of xylanase for the preparation of xylose by enzymatic degradation,and the hydrolysis process was optimized by response surface methodologies（RSMs）.According to the results of single factor experiment,five parameters which had great influence on the yield of xylose were selected for 53 full factorial experiment designed by Box-Benhnken central composite method.The obtained optimum conditions of enzymatic hydrolysis of corncob pretreated by steam explosion were：xylanase amount 13.05U/g（based on corncob）,water-feed ratio 13.2∶1（mL∶g）,enzymatic time 5h,temperature 47.3℃,pH value 5.84.Under the optimum conditions,the yield of xylose reached upto 83.24%.

关 键 词：响应面法 玉米芯 酶解 木糖 

分 类 号：TQ351.0[化学工程]