菌株Streptomyces S_(90)抗菌物质的初步鉴定及抑菌作用机制研究  

Primary Identification and Its Mechanism of the Antifungal Substances from Streptomyces S_(90)

作　　者：孙泽洋[1] 吴元华[1] 赵秀香[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866

出　　处：《湖北农业科学》2010年第10期2442-2444,共3页Hubei Agricultural Sciences

基　　金：辽宁省科技厅资助项目(2006215004);辽宁省教育厅资助项目(20060783);高等学校博士点专项基金(20070157010)

摘　　要：采用Doskochilova溶剂系统层析、pH纸层析和纸电泳测定表明:菌株StreptomycesS90发酵液中具有抑菌活性的物质为一种单组分碱性水溶性抗生素。采用大孔树脂吸附法提取得到了该抗菌物质的粗提物,研究其对烟草赤星病菌的作用机制表明,当抗菌物质浓度为1.6mg/mL时,对菌丝生长和孢子萌发的抑制率分别达到82.06%和78.80%;随着各处理浓度的增加,抗菌物质使病菌菌丝细胞膜电导率和丙二醛含量变化均增加;抗菌物质对病菌菌丝细胞壁组成物质几丁质没有影响。By means of antibiotic special identification including paper chromatography of Doskochilova solvent systems,pH chromatography and paper electrophoresis,a basic alkaline water soluble antibiotic was found in the broth of Streptomyces S90. The crude extract was extracted by macroporous adsorption resin. The antibiotic activities against Alternaria alternata showed the inhibition rates of mycelial growth and spore germination inhibition reached 82.06% and 78.80% respectively when the crude extract content was 1.6 mg / mL in medium. On the other hand, the increased conductivity of hyphal cell membrane and malonic dialdehyde were observed when increased the concentration of each treatment, however, there was no obvious effect on the chitin in hyphal cell wall.

关 键 词：STREPTOMYCES S90 抗菌物质 烟草赤星病菌 抑菌作用 

分 类 号：S182[农业科学—农业基础科学] S482.28