八倍体小冰麦及其亲本种子醇溶蛋白和高分子量麦谷蛋白亚基的研究  

Study of Gliadin and High Molecular Weight Glutenin in Octoploid Wheat Wheatgrass and Its Wheat Parents

作　　者：焦明大[1] 韩方普[1] 何孟元[1] 郝水[1] 张延滨[2] 祁适雨[2] 

机构地区：[1]东北师范大学遗传与细胞研究所 [2]黑龙江省农业科学院育种研究所

出　　处：《Acta Botanica Sinica》1999年第4期405-408,共4页Acta Botanica Sinica（植物学报：英文版）

摘　　要：对５个八倍体小冰麦种子醇溶蛋白和高分子量麦谷蛋白亚基的电泳谱带进行了分析，结果表明：八倍体小冰麦中１和中２的电泳谱带基本相同，中３、中４、中５的电泳谱带基本相同，但完全不同于中１和中２的类型。八倍体小冰麦中１和中２同天蓝冰草（Ａｇｒｏｐｙｒｏｎｉｎｔｅｒｍｅｄｉｕｍ（Ｈｏｓｔ）Ｐ．Ｂ．＝Ｅｌｙｔｒｉｇｉａｉｎｔｅｒｍｅｄｉａ（Ｈｏｓｔ）Ｎｅｖｓｋｉ＝Ｔｈｉｎｏｐｙｒｕｍｉｎｔｅｒｍｅｄｉｕｍ（Ｈｏｓｔ）ＢａｒｋｗａｒｔｈａｎｄＤｅｗｅｙ）在高分子量麦谷蛋白亚基上存在一条相同的谱带，在醇溶蛋白谱带上出现了小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）和冰草均没有的带型。中３、中４、中５在醇溶蛋白谱带上具有一条冰草×染色体组的特征谱带，其基因表达程度同冰草类似。从５个八倍体小冰麦种子醇溶蛋白和高分子量麦谷蛋白亚基的电泳图谱结果，分析了八倍体小冰麦染色体组构成及亲本来源，并探讨了八倍体小冰麦在优质麦育种过程中的价值。The electrophoretograms of gliadin and high molecular weight (HMW) glutenin in the five octoploid amphiploids of wheat wheatgrass were analyzed. The results indicated that the octoploid amphiploids of wheat (Triticum aestivum L.) wheatgrass(Agropyron intermedium (Host) P.B.=Elytrigia intermedia (Host) Nevski=Thinopyrum intermedium (Host) Barkwarth and Dewey) Zhong 1 and Zhong 2 had the same band patterns, and then the octoploid amphiploids of wheat wheatgrass Zhong 3, Zhong 4 and Zhong 5 had the same band patterns which were different from Zhong 1 and Zhong 2. One band pattern of HMW glutenin which lack in wheat parents were occurred in Zhong 1, Zhong 2 and wheatgrass. New band patterns which were different from both of wheat and wheatgrass may also occur in Zhong 1 and Zhong 2. The authors found that the genome X of wheatgrass expressed one specific band pattern occurred in Zhong 3, Zhong 4 and Zhong 5. Authors analyzed the genomic constitution and wheat parents of the five octoploid amphiploids of wheat wheatgrass by their band patterns of gliadin and HMW glutenin. Furthermore, the potential significance of the octoploid amphiploids of wheat wheatgrass for wheat breeding was discussed.

关 键 词：小麦 醇溶蛋白 麦谷蛋白亚基 八倍体 小冰麦 

分 类 号：S512.103.5[农业科学—作物学]