大肠杆菌发酵重组人干扰素-α2a的高密度、高表达  

THE HIGH DENSITY AND HIGH EXPRESSION LEVEL FERMENTATION OF RECOMBINANT HUMAN INTERFERON-α2a PRODUCING E. coli STRAIN

作　　者：邹钟诚[1] 侯剑英[1] 王增学[1] 苏冬梅[1] 

机构地区：[1]沈阳三生制药股份有限公司,110141

出　　处：《微生物学杂志》1999年第1期24-26,共3页Journal of Microbiology

摘　　要：利用NBS公司的BioFloⅢ发酵罐，采用培养基流加的方式培养表达人干扰素的E．coli工程菌，结果湿菌重达128g／L，干扰素活性达98×1010U／L发酵液。An engineering recominant human interferon producing strain of E. coli was culturedin a 5L BioFlo III fermentor (NBS) by means of now - feeding into the medium during thefermentation. The results were 128g/L of cell weight and 9. 8 ×1010 U/L of interferon ac-tivity respectively.

关 键 词：重组 干扰素 大肠杆菌 高密度 高表达 发酵 

分 类 号：TQ929.1[轻工技术与工程—发酵工程] Q784[生物学—分子生物学]